Cancer cell culture

Table of Contents

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   Book Overview
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   Chapter 1 Cancer Biology
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   Chapter 2 Principles of cancer cell culture.
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   Chapter 3 Storage of Cell Lines
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   Chapter 4 Characterization and Authentication of Cancer Cell Lines: An Overview
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   Chapter 5 Online Verification of Human Cell Line Identity by STR DNA Typing
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   Chapter 6 Cytogenetic Analysis of Cancer Cell Lines
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   Chapter 7 Cell Culture Contamination
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   Chapter 8 Detecting mycoplasma contamination in cell cultures by polymerase chain reaction.
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    Chapter 9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics
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    Chapter 10 Quality Assurance and Good Laboratory Practice
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    Chapter 11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning
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    Chapter 12 Isolation and Culture of Colon Cancer Cells and Cell Lines
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    Chapter 13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
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    Chapter 14 Isolation and Culture of Squamous Cell Carcinoma Lines
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    Chapter 15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines
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    Chapter 16 Establishment and Culture of Leukemia–Lymphoma Cell Lines
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    Chapter 17 Isolation of Inflammatory Cells from Human Tumours
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    Chapter 18 Isolation of Endothelial Cells from Human Tumors
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    Chapter 19 Cellular Chemosensitivity Assays: An Overview
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    Chapter 20 Cell sensitivity assays: the MTT assay.
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    Chapter 21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
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    Chapter 22 Differential Staining Cytotoxicity Assay: A Review
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    Chapter 23 Real-time cytotoxicity assays.
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    Chapter 24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
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    Chapter 25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay
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    Chapter 26 Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR
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    Chapter 27 Cell Migration and Invasion Assays
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    Chapter 28 Angiogenesis Assays
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    Chapter 29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
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    Chapter 30 Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines
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    Chapter 31 Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transcriptase Polymerase Chain Reaction
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    Chapter 32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins
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    Chapter 33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated Immunotherapy
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    Chapter 34 Analysis of Drug Interactions
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    Chapter 35 Transfection and DNA-Mediated Gene Transfer
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    Chapter 36 Drug Design and Testing: Profiling of Antiproliferative Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]-Thymidine Incorporation Assay
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    Chapter 37 Feeder Layers: Co-culture with Nonneoplastic Cells
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    Chapter 38 Xenotransplantation of Breast Cancers