Report for: Glycosaminoglycans

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Chapter title	Cell substrate patterning with glycosaminoglycans to study their biological roles in the central nervous system.
Chapter number	35
Book title	Glycosaminoglycans
Published in	Methods in molecular biology, January 2015
DOI	10.1007/978-1-4939-1714-3_35
Pubmed ID	25325972
Book ISBNs	978-1-4939-1713-6, 978-1-4939-1714-3
Authors	Tony W Hsiao, Vimal P Swarup, Colin D Eichinger, Vladimir Hlady, Tony W. Hsiao, Vimal P. Swarup, Colin D. Eichinger, Hsiao, Tony W., Swarup, Vimal P., Eichinger, Colin D., Hlady, Vladimir
Abstract	Microcontact printing (μCP) based techniques have been developed for creating cell culture substrates with discrete placement of CNS-expressed molecules. These substrates can be used to study various components of the complex molecular environment in the central nervous system (CNS) and related cellular responses. Macromolecules such as glycosaminoglycans (GAGs), proteoglycans (PGs), or proteins are amenable to printing. Detailed protocols for both adsorption based as well as covalent reaction printing of cell culture substrates are provided. By utilizing a modified light microscope, precise placement of two or more types of macromolecules by sequential μCP can be used to create desired spatial arrangements containing multicomponent PG, GAG, and protein surface patterns for studying CNS cell behavior. Examples of GAG stripe assays for neuronal pathfinding and directed outgrowth, and dot gradients of PG + laminin for astrocyte migration studies are provided.

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Geographical breakdown

Country	Count	As %
United States	1	50%
Unknown	1	50%

Demographic breakdown

Type	Count	As %
Members of the public	2	100%

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The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

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Country	Count	As %
Unknown	1	100%

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Readers by professional status	Count	As %
Professor > Associate Professor	1	100%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	1	100%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 June 2015.

All research outputs

#14,787,304

of 22,766,595 outputs

Outputs from Methods in molecular biology

#4,676

of 13,090 outputs

Outputs of similar age

#197,789

of 352,895 outputs

Outputs of similar age from Methods in molecular biology

#295

of 996 outputs

Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.

So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 59% of its peers.

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We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.

Glycosaminoglycans

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