Chapter title |
Detection of Carbapenemases Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay.
|
---|---|
Chapter number | 9 |
Book title |
Sepsis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1776-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1775-4, 978-1-4939-1776-1
|
Authors |
Jaroslav Hrabák, Hrabák, Jaroslav |
Abstract |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identification of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confirm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 23% |
Australia | 1 | 8% |
Netherlands | 1 | 8% |
Unknown | 8 | 62% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 62% |
Scientists | 5 | 38% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 22% |
Researcher | 5 | 19% |
Other | 4 | 15% |
Student > Postgraduate | 2 | 7% |
Student > Ph. D. Student | 2 | 7% |
Other | 4 | 15% |
Unknown | 4 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 22% |
Agricultural and Biological Sciences | 4 | 15% |
Immunology and Microbiology | 3 | 11% |
Chemistry | 3 | 11% |
Veterinary Science and Veterinary Medicine | 2 | 7% |
Other | 4 | 15% |
Unknown | 5 | 19% |