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Spliceosomal Pre-mRNA Splicing

Overview of attention for book
Cover of 'Spliceosomal Pre-mRNA Splicing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Pre-mRNA Splicing Reaction.
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    Chapter 2 Diversity and Evolution of Spliceosomal Systems
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    Chapter 3 Mechanisms of Spliceosomal Assembly
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    Chapter 4 Alternative Pre-mRNA Splicing
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    Chapter 5 Regulation of Alternative Pre-mRNA Splicing.
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    Chapter 6 Introduction to Cotranscriptional RNA Splicing
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    Chapter 7 Chromatin and Splicing
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    Chapter 8 Preparation of splicing competent nuclear extracts.
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    Chapter 9 Preparation of Yeast Whole Cell Splicing Extract
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    Chapter 10 Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase
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    Chapter 11 In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract.
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    Chapter 12 Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract.
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    Chapter 13 In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation
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    Chapter 14 Isolation and Accumulation of Spliceosomal Assembly Intermediates
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    Chapter 15 Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function
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    Chapter 16 Expression and Purification of Splicing Proteins from Mammalian Cells
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    Chapter 17 Single molecule approaches for studying spliceosome assembly and catalysis.
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    Chapter 18 Cell-Based Splicing of Minigenes
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    Chapter 19 Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA
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    Chapter 20 Antisense Methods to Modulate Pre-mRNA Splicing
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    Chapter 21 Using Yeast Genetics to Study Splicing Mechanisms
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    Chapter 22 Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR
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    Chapter 23 Chromatin Immunoprecipitation Approaches to Determine Co-transcriptional Nature of Splicing
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    Chapter 24 Computational approaches to mine publicly available databases.
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    Chapter 25 Approaches to link RNA secondary structures with splicing regulation
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    Chapter 26 Methods to study splicing from high-throughput RNA sequencing data.
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    Chapter 27 Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq.
  29. Altmetric Badge
    Chapter 28 Predicting Alternative Splicing
Attention for Chapter 17: Single molecule approaches for studying spliceosome assembly and catalysis.
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  • Good Attention Score compared to outputs of the same age and source (74th percentile)

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Chapter title
Single molecule approaches for studying spliceosome assembly and catalysis.
Chapter number 17
Book title
Spliceosomal Pre-mRNA Splicing
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-980-2_17
Pubmed ID
24549668
Book ISBNs
978-1-62703-979-6, 978-1-62703-980-2
Authors

Anderson EG, Hoskins AA, Eric G. Anderson, Aaron A. Hoskins

Abstract

Single molecule assays of splicing and spliceosome assembly can provide unique insights into pre-mRNA processing that complement other technologies. Key to these experiments is the fabrication of fluorescent molecules (pre-mRNAs and spliceosome components) and passivated glass slides for each experiment. Here we describe how to produce fluorescent RNAs by splinted RNA ligation and fluorescent spliceosome subunits by SNAP-tagging proteins in cell lysate. We then depict how to passivate glass slides with polyethylene glycol for use on an inverted microscope with objective-based total internal reflection fluorescence (TIRF) optics. Finally, we describe how to tether the pre-mRNA onto the passivated slide surface and introduce the SNAP-tagged cell lysate for analysis of spliceosome assembly by single molecule fluorescence.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Professor > Associate Professor 2 20%
Researcher 2 20%
Student > Bachelor 1 10%
Other 1 10%
Other 0 0%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 50%
Biochemistry, Genetics and Molecular Biology 2 20%
Chemistry 1 10%
Engineering 1 10%
Unknown 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2014.
All research outputs
#15,448,846
of 22,958,253 outputs
Outputs from Methods in molecular biology
#5,372
of 13,137 outputs
Outputs of similar age
#135,042
of 225,038 outputs
Outputs of similar age from Methods in molecular biology
#28
of 137 outputs
Altmetric has tracked 22,958,253 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 225,038 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 137 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.

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