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Recombinant protein production in yeast : methods and protocols

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Cover of 'Recombinant protein production in yeast : methods and protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Optimising Yeast as a Host for Recombinant Protein Production (Review)
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    Chapter 2 Which Yeast Species Shall I Choose? Saccharomyces cerevisiae Versus Pichia pastoris (Review)
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    Chapter 3 Preparation of Pichia pastoris Expression Plasmids
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    Chapter 4 Preparation of Saccharomyces cerevisiae Expression Plasmids
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    Chapter 5 Codon Optimisation for Heterologous Gene Expression in Yeast
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    Chapter 6 Yeast Transformation to Generate High-Yielding Clones
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    Chapter 7 Screening for High-Yielding Pichia pastoris Clones: The Production of G Protein-Coupled Receptors as a Case Study
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    Chapter 8 Screening for High-Yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
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    Chapter 9 The Effect of Antifoam Addition on Protein Production Yields
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    Chapter 10 Setting Up a Bioreactor for Recombinant Protein Production in Yeast
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    Chapter 11 The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (Review)
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    Chapter 12 Online Analysis and Process Control in Recombinant Protein Production (Review)
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    Chapter 13 Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Offline Estimations of Dry Cell Mass and Cell Counts
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    Chapter 14 Online Monitoring of Biomass Accumulation in Recombinant Yeast Cultures
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    Chapter 15 Optimising Pichia pastoris Induction
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    Chapter 16 Optimizing Saccharomyces cerevisiae Induction Regimes
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    Chapter 17 Large-Scale Production of Membrane Proteins in Pichia pastoris : The Production of G Protein-Coupled Receptors as a Case Study
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    Chapter 18 Large-Scale Production of Membrane Proteins in Saccharomyces cerevisiae : Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
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    Chapter 19 Large-Scale Production of Secreted Proteins in Pichia pastoris
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    Chapter 20 Disruption of Yeast Cells to Isolate Recombinant Proteins
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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