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Recombinant protein production in yeast : methods and protocols

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Cover of 'Recombinant protein production in yeast : methods and protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Optimising Yeast as a Host for Recombinant Protein Production (Review)
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    Chapter 2 Which Yeast Species Shall I Choose? Saccharomyces cerevisiae Versus Pichia pastoris (Review)
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    Chapter 3 Preparation of Pichia pastoris Expression Plasmids
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    Chapter 4 Preparation of Saccharomyces cerevisiae Expression Plasmids
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    Chapter 5 Codon Optimisation for Heterologous Gene Expression in Yeast
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    Chapter 6 Yeast Transformation to Generate High-Yielding Clones
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    Chapter 7 Screening for High-Yielding Pichia pastoris Clones: The Production of G Protein-Coupled Receptors as a Case Study
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    Chapter 8 Screening for High-Yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
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    Chapter 9 The Effect of Antifoam Addition on Protein Production Yields
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    Chapter 10 Setting Up a Bioreactor for Recombinant Protein Production in Yeast
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    Chapter 11 The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (Review)
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    Chapter 12 Online Analysis and Process Control in Recombinant Protein Production (Review)
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    Chapter 13 Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Offline Estimations of Dry Cell Mass and Cell Counts
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    Chapter 14 Online Monitoring of Biomass Accumulation in Recombinant Yeast Cultures
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    Chapter 15 Optimising Pichia pastoris Induction
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    Chapter 16 Optimizing Saccharomyces cerevisiae Induction Regimes
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    Chapter 17 Large-Scale Production of Membrane Proteins in Pichia pastoris : The Production of G Protein-Coupled Receptors as a Case Study
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    Chapter 18 Large-Scale Production of Membrane Proteins in Saccharomyces cerevisiae : Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
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    Chapter 19 Large-Scale Production of Secreted Proteins in Pichia pastoris
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    Chapter 20 Disruption of Yeast Cells to Isolate Recombinant Proteins
Attention for Chapter 10: Setting Up a Bioreactor for Recombinant Protein Production in Yeast
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Chapter title
Setting Up a Bioreactor for Recombinant Protein Production in Yeast
Chapter number 10
Book title
Recombinant Protein Production in Yeast
Published by
Humana Press, January 2012
DOI 10.1007/978-1-61779-770-5_10
Book ISBNs
978-1-61779-769-9, 978-1-61779-770-5
Authors

Sarah J. Routledge, Michelle Clare, Routledge, Sarah J., Clare, Michelle

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 3%
Unknown 33 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 24%
Student > Master 5 15%
Student > Ph. D. Student 4 12%
Student > Bachelor 3 9%
Professor 3 9%
Other 6 18%
Unknown 5 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 32%
Biochemistry, Genetics and Molecular Biology 10 29%
Social Sciences 3 9%
Engineering 2 6%
Unspecified 1 3%
Other 2 6%
Unknown 5 15%