| Chapter title |
Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking
|
|---|---|
| Chapter number | -896032103 |
| Book title |
PCR Protocols
|
| Published in |
Methods in molecular biology, January 1993
|
| DOI | 10.1385/0-89603-244-2:339 |
| Pubmed ID | |
| Book ISBNs |
978-0-89603-244-6, 978-1-59259-502-0
|
| Authors |
Venkatakrishna Shyamala, Giovanna Ferro-Luzzi Ames, Shyamala, Venkatakrishna, Ferro-Luzzi Ames, Giovanna |
| Abstract |
The polymerase chain reaction (PCR) is used for selective amplification of DNA fragments from both prokaryotes and eukaryotes (1-3). The only requirement for amplification is that the sequence of the extremities of the DNA fragment to be amplified be known (4). This places a limitation on the use of PCR in the amplification of adjacent unknown regions. We have developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only (5). This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 18% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Master | 5 | 15% |
| Researcher | 3 | 9% |
| Student > Postgraduate | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 10 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 36% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Medicine and Dentistry | 2 | 6% |
| Immunology and Microbiology | 1 | 3% |
| Social Sciences | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 11 | 33% |