↓ Skip to main content

Kidney Development

Overview of attention for book
Cover of 'Kidney Development'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Dissecting and Culturing and Imaging the Mouse Urogenital System
  3. Altmetric Badge
    Chapter 2 In Vitro Culture of Embryonic Kidney Rudiments and Isolated Ureteric Buds
  4. Altmetric Badge
    Chapter 3 In Vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud Recombination
  5. Altmetric Badge
    Chapter 4 Live Imaging of the Developing Mouse Mesonephros
  6. Altmetric Badge
    Chapter 5 Organotypic Culture of the Urogenital Tract
  7. Altmetric Badge
    Chapter 6 Live Imaging Kidney Development in Zebrafish
  8. Altmetric Badge
    Chapter 7 Analysis of 3D Branching Pattern: Hematoxylin and Eosin Method
  9. Altmetric Badge
    Chapter 8 Three-Dimensional Imaging of Fetal Mouse Kidneys
  10. Altmetric Badge
    Chapter 9 Analysis of Native Kidney Structures in Three Dimensions
  11. Altmetric Badge
    Chapter 10 Estimating Nephron Number in the Developing Kidney Using the Physical Disector/Fractionator Combination
  12. Altmetric Badge
    Chapter 11 An Immunofluorescence Method to Analyze the Proliferation Status of Individual Nephron Segments in the Xenopus Pronephric Kidney
  13. Altmetric Badge
    Chapter 12 Dissociation of Embryonic Kidney Followed by Re-aggregation as a Method for Chimeric Analysis.
  14. Altmetric Badge
    Chapter 13 Analysis of Migration in Primary Ureteric Bud Epithelial Cells
  15. Altmetric Badge
    Chapter 14 Investigating Primary Cilia in Cultured Metanephric Mesenchymal Cells
  16. Altmetric Badge
    Chapter 15 Making immortalized cell lines from embryonic mouse kidney.
  17. Altmetric Badge
    Chapter 16 Engineered Tissues to Quantify Collective Cell Migration During Morphogenesis
  18. Altmetric Badge
    Chapter 17 Access and Use of the GUDMAP Database of Genitourinary Development.
  19. Altmetric Badge
    Chapter 18 Isolation of High Quality RNA from Embryonic Kidney and Cells
  20. Altmetric Badge
    Chapter 19 Laser Capture
  21. Altmetric Badge
    Chapter 20 Kidney Development
  22. Altmetric Badge
    Chapter 21 Detection of β-Galactosidase Activity: X-gal Staining.
  23. Altmetric Badge
    Chapter 22 Fluorescent Immunolabeling of Embryonic Kidney Samples
  24. Altmetric Badge
    Chapter 23 Immunohistochemical Staining of dpERK Staining During Early Kidney Development
  25. Altmetric Badge
    Chapter 24 Sensing BMP Pathway Activity by Immune Detection of Phosphorylated R-Smad Proteins in Mouse Embryonic Kidney
  26. Altmetric Badge
    Chapter 25 Analysis of In Vivo Transcription Factor Recruitment by Chromatin Immunoprecipitation of Mouse Embryonic Kidney
  27. Altmetric Badge
    Chapter 26 siRNA-Mediated RNA Interference in Embryonic Kidney Organ Culture.
  28. Altmetric Badge
    Chapter 27 Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture
  29. Altmetric Badge
    Chapter 28 Microinjection into the Lumen of the Ureteric Tree
  30. Altmetric Badge
    Chapter 29 Renal Delivery of Adenovirus and Antisense Oligonucleotides in Rats by Retrograde Renal Vein Injection
  31. Altmetric Badge
    Chapter 30 Estimating Total Nephron Number in the Adult Kidney Using the Physical Disector/Fractionator Combination
  32. Altmetric Badge
    Chapter 31 Assessing Urinary Tract Defects in Mice: Methods to Detect the Presence of Vesicoureteric Reflux and Urinary Tract Obstruction
  33. Altmetric Badge
    Chapter 32 Ischemia–Reperfusion Injury of the Mouse Kidney
  34. Altmetric Badge
    Chapter 33 Variable partial unilateral ureteral obstruction and its release in the neonatal and adult mouse.
  35. Altmetric Badge
    Chapter 34 Urinary Diversion via Cutaneous Vesicostomy in the Megabladder Mouse
  36. Altmetric Badge
    Chapter 35 Ultrasound Imaging of the Murine Kidney
  37. Altmetric Badge
    Chapter 36 Erratum to: Access and Use of the GUDMAP Database of Genitourinary Development
Attention for Chapter 21: Detection of β-Galactosidase Activity: X-gal Staining.
Altmetric Badge

Mentioned by

facebook
1 Facebook page

Citations

dimensions_citation
8 Dimensions

Readers on

mendeley
212 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Detection of β-Galactosidase Activity: X-gal Staining.
Chapter number 21
Book title
Kidney Development
Published in
Methods in molecular biology, May 2012
DOI 10.1007/978-1-61779-851-1_21
Pubmed ID
Book ISBNs
978-1-61779-850-4, 978-1-61779-851-1
Authors

Burn SF, Sally F. Burn, Burn, Sally F.

Abstract

X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked into the gene of interest or placed under the control of regulatory elements corresponding to the gene of interest. Expression is marked by a dark blue stain and can be detected at the single cell level, providing a robust visual readout of gene expression in the developing kidney. Here, we describe the methodology, applications, and limitations of this technique.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 212 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
Portugal 1 <1%
Unknown 210 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 82 39%
Student > Master 19 9%
Student > Ph. D. Student 18 8%
Researcher 8 4%
Other 6 3%
Other 10 5%
Unknown 69 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 67 32%
Agricultural and Biological Sciences 31 15%
Pharmacology, Toxicology and Pharmaceutical Science 6 3%
Medicine and Dentistry 6 3%
Chemistry 6 3%
Other 21 10%
Unknown 75 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2014.
All research outputs
#20,238,443
of 22,765,347 outputs
Outputs from Methods in molecular biology
#9,866
of 13,090 outputs
Outputs of similar age
#149,243
of 165,202 outputs
Outputs of similar age from Methods in molecular biology
#38
of 42 outputs
Altmetric has tracked 22,765,347 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 165,202 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.