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Mendeley readers
Chapter title |
In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
|
---|---|
Chapter number | 8 |
Book title |
Cancer Stem Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7401-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7400-9, 978-1-4939-7401-6
|
Authors |
Vijayalakshmi Rajendran, Mayur Vilas Jain |
Abstract |
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies. |
Mendeley readers
The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 123 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 16% |
Student > Master | 15 | 12% |
Student > Bachelor | 13 | 11% |
Researcher | 9 | 7% |
Student > Doctoral Student | 9 | 7% |
Other | 7 | 6% |
Unknown | 50 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 36 | 29% |
Agricultural and Biological Sciences | 12 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 5% |
Medicine and Dentistry | 5 | 4% |
Unspecified | 3 | 2% |
Other | 9 | 7% |
Unknown | 52 | 42% |