| Chapter title |
Hemorrhagic Fever Virus Budding Studies
|
|---|---|
| Chapter number | 15 |
| Book title |
Hemorrhagic Fever Viruses
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-6981-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6980-7, 978-1-4939-6981-4
|
| Authors |
Ronald N. Harty |
| Abstract |
Independent expression of the VP40 or Z matrix proteins of filoviruses (marburgviruses and ebolaviruses) and arenaviruses (Lassa fever and Junín), respectively, gives rise to the production and release of virus-like particles (VLPs) that are morphologically identical to infectious virions. We can detect and quantify VLP production and egress in mammalian cells by transient transfection, SDS-PAGE, Western blotting, and live cell imaging techniques such as total internal reflection fluorescence (TIRF) microscopy. Since the VLP budding assay accurately mimics budding of infectious virus, this BSL-2 assay is safe and useful for the interrogation of both viral and host determinants required for budding and can be used as an initial screen to identify and validate small molecule inhibitors of virus release and spread. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 19% |
| Student > Ph. D. Student | 6 | 19% |
| Student > Bachelor | 5 | 16% |
| Student > Postgraduate | 2 | 6% |
| Student > Master | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 9 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 25% |
| Agricultural and Biological Sciences | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Immunology and Microbiology | 3 | 9% |
| Nursing and Health Professions | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 11 | 34% |