| Chapter title |
Protocol for the Production of a Vaccine Against Argentinian Hemorrhagic Fever
|
|---|---|
| Chapter number | 24 |
| Book title |
Hemorrhagic Fever Viruses
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-6981-4_24 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6980-7, 978-1-4939-6981-4
|
| Authors |
Ana María Ambrosio, Mauricio Andrés Mariani, Andrea Soledad Maiza, Graciela Susana Gamboa, Sebastián Edgardo Fossa, Alejando Javier Bottale, Ambrosio, Ana María, Mariani, Mauricio Andrés, Maiza, Andrea Soledad, Gamboa, Graciela Susana, Fossa, Sebastián Edgardo, Bottale, Alejando Javier |
| Abstract |
Argentinian hemorrhagic Fever (AHF) is a febrile, acute disease caused by Junín virus (JUNV), a member of the Arenaviridae. Different approaches to obtain an effective antigen to prevent AHF using complete live or inactivated virus, as well as molecular constructs, have reached diverse development stages. This chapter refers to JUNV live attenuated vaccine strain Candid #1, currently used in Argentina to prevent AHF. A general standardized protocol used at Instituto Nacional de Enfermedades Virales Humanas (Pergamino, Pcia. Buenos Aires, Argentina) to manufacture the tissue culture derived Candid #1 vaccine is described. Intermediate stages like viral seeds and cell culture bank management, bulk vaccine manufacture, and finished product processing are also separately presented in terms of Production and Quality Control/Quality Assurance requirements, under the Adminitracion Nacional de Medicamentos, Alimentos y Tecnología Medica (ANMAT), the Argentine national regulatory authority. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 24% |
| Student > Master | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Student > Ph. D. Student | 1 | 5% |
| Unknown | 12 | 57% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
| Social Sciences | 2 | 10% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 11 | 52% |