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Mendeley readers
Chapter title |
Detection of Virus-Antibody Immune Complexes in Secondary Dengue Virus Infection
|
---|---|
Chapter number | 25 |
Book title |
Hemorrhagic Fever Viruses
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-6981-4_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6980-7, 978-1-4939-6981-4
|
Authors |
Meng Ling Moi, Tomohiko Takasaki, Ichiro Kurane |
Abstract |
It has been reported that virus-antibody immune complexes formed during secondary dengue virus infection are associated with increased disease severity. Here, we describe the details of a plaque titration method that uses FcγR -expressing BHK cells to detect and quantify infectious virus-immune complexes and a quantitative real-time PCR method for the quantification of virus genome in patients with secondary dengue infection. These methods detect both viruses in free-form and in immune complexes in patients with dengue infection, and are useful for determining viremia levels and patterns that better reflect in vivo conditions. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 28% |
Student > Bachelor | 3 | 17% |
Student > Master | 3 | 17% |
Student > Ph. D. Student | 1 | 6% |
Other | 1 | 6% |
Other | 2 | 11% |
Unknown | 3 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 5 | 28% |
Biochemistry, Genetics and Molecular Biology | 4 | 22% |
Medicine and Dentistry | 2 | 11% |
Agricultural and Biological Sciences | 2 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 22% |