Chapter title |
Studies of Lassa Virus Cell Entry
|
---|---|
Chapter number | 9 |
Book title |
Hemorrhagic Fever Viruses
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-6981-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6980-7, 978-1-4939-6981-4
|
Authors |
Antonella Pasquato, Antonio Herrador Fernandez, Stefan Kunz, Pasquato, Antonella, Fernandez, Antonio Herrador, Kunz, Stefan |
Abstract |
Host cell entry is the first and most fundamental step of every virus infection and represents a major barrier for zoonotic transmission and viral emergence. Targeting viral entry appears further as a promising strategy for therapeutic intervention. Several cellular receptors have been identified for Lassa virus, including dystroglycan, TAM receptor tyrosine kinases, and C-type lectins. Upon receptor binding, LASV enters the host cell via a largely unknown clathrin- and dynamin-independent endocytotic pathway that delivers the virus to late endosomes, where fusion occurs after engagement of a second, intracellular receptor, the late endosomal/lysosomal resident protein LAMP1. Here, we describe a series of experimental approaches to investigate LASV cell entry and to test candidate inhibitors for their action at this early and decisive step of infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 33% |
Student > Master | 4 | 19% |
Student > Ph. D. Student | 2 | 10% |
Student > Postgraduate | 2 | 10% |
Professor > Associate Professor | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 19% |
Immunology and Microbiology | 3 | 14% |
Environmental Science | 2 | 10% |
Medicine and Dentistry | 2 | 10% |
Agricultural and Biological Sciences | 2 | 10% |
Other | 3 | 14% |
Unknown | 5 | 24% |