Chapter title |
Establishment of Bisegmented and Trisegmented Reverse Genetics Systems to Generate Recombinant Pichindé Viruses
|
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Chapter number | 19 |
Book title |
Hemorrhagic Fever Viruses
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-6981-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6980-7, 978-1-4939-6981-4
|
Authors |
Rekha Dhanwani, Qinfeng Huang, Shuiyun Lan, Yanqing Zhou, Junjie Shao, Yuying Liang, Hinh Ly |
Abstract |
Pichindé virus (PICV), isolated from rice rats in Colombia, South America, is an enveloped arenavirus with a bisegmented RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-dependent RNA polymerase, whereas the small (S) genomic segment encodes the nucleoprotein (NP) and the glycoprotein (GPC). This article describes the successful development of reverse genetics systems to generate recombinant PICV with either a bisegmented or trisegmented genome. We have successfully demonstrated that these systems can generate high-titered and genetically stable replication-competent viruses from plasmid transfection into appropriate cell lines. These systems demonstrate the power and versatility of reverse genetic technology to generate recombinant arenaviruses for use in pathogenesis studies and as new viral vaccine vectors. |
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Unknown | 1 | 100% |
Demographic breakdown
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 13 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 31% |
Researcher | 2 | 15% |
Lecturer | 1 | 8% |
Other | 1 | 8% |
Student > Master | 1 | 8% |
Other | 1 | 8% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 3 | 23% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Chemical Engineering | 1 | 8% |
Computer Science | 1 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |