Chapter title |
Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application System
|
---|---|
Chapter number | 8 |
Book title |
NMDA Receptors
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7321-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7320-0, 978-1-4939-7321-7
|
Authors |
Andrei Rozov, David Jappy |
Abstract |
A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261-287, 1992; Jonas and Sakmann, J Physiol 455:143-171, 1992). |
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