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NMDA Receptors

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Cover of 'NMDA Receptors'

Table of Contents

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    Book Overview
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    Chapter 1 NMDA Receptors in the Central Nervous System
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    Chapter 2 Quantification of NMDAR Subunit Genes Expression by qRT-PCR
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    Chapter 3 Genetic and Functional Analysis of GRIN2A in Tumor Samples
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    Chapter 4 Detection of NMDARs Antibodies in Encephalitis
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    Chapter 5 Magnetofection™ of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells
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    Chapter 6 Transfection in Primary Cultured Neuronal Cells
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    Chapter 7 Selective Cell-Surface Expression of Triheteromeric NMDA Receptors
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    Chapter 8 Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application System
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    Chapter 9 GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging
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    Chapter 10 Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers
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    Chapter 11 Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations
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    Chapter 12 Electrophysiological Investigation of NMDA Current Properties in Brain Slices
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    Chapter 13 Analysis of Functional NMDA Receptors in Astrocytes
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    Chapter 14 GluNs Detection and Functions in Microglial Cells
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    Chapter 15 NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detection
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    Chapter 16 NMDA Receptors as Voltage Sensors
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    Chapter 17 Development of a Computational Approach/Model to Explore NMDA Receptors Functions
Attention for Chapter 8: Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application System
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Chapter title
Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application System
Chapter number 8
Book title
NMDA Receptors
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7321-7_8
Pubmed ID
Book ISBNs
978-1-4939-7320-0, 978-1-4939-7321-7
Authors

Andrei Rozov, David Jappy

Abstract

A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261-287, 1992; Jonas and Sakmann, J Physiol 455:143-171, 1992).

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 75%
Professor 1 25%
Readers by discipline Count As %
Neuroscience 2 50%
Pharmacology, Toxicology and Pharmaceutical Science 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2018.
All research outputs
#20,449,496
of 23,005,189 outputs
Outputs from Methods in molecular biology
#9,941
of 13,159 outputs
Outputs of similar age
#356,165
of 421,224 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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