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Regulatory Non-Coding RNAs

Overview of attention for book
Cover of 'Regulatory Non-Coding RNAs'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Diverse Functions and Mechanisms of Mammalian Long Noncoding RNAs.
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    Chapter 2 Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.
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    Chapter 3 A Biochemical Approach to Identify Direct MicroRNA Targets.
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    Chapter 4 Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-microRNAs.
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    Chapter 5 Identification and Validation of miRNA Target Sites Within Nontraditional miRNA Targets.
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    Chapter 6 Fractionation of Non-polyadenylated and Ribosomal-Free RNAs from Mammalian Cells
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    Chapter 7 Affinity Purification of Long Noncoding RNA-Protein Complexes from Formaldehyde Cross-Linked Mammalian Cells.
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    Chapter 8 RNA Pulldown Protocol for In Vitro Detection and Identification of RNA-Associated Proteins
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    Chapter 9 nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale.
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    Chapter 10 Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization
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    Chapter 11 RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells
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    Chapter 12 Locating RNAs In Situ with FISH-STIC Probes.
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    Chapter 13 Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides
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    Chapter 14 On Using Magnesium and Potassium Ions in RNA Experiments
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    Chapter 15 Regulatory Non-Coding RNAs
Attention for Chapter 4: Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-microRNAs.
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About this Attention Score

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  • Good Attention Score compared to outputs of the same age and source (66th percentile)

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Chapter title
Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-microRNAs.
Chapter number 4
Book title
Regulatory Non-Coding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1369-5_4
Pubmed ID
25240885
Book ISBNs
978-1-4939-1368-8, 978-1-4939-1369-5
Authors

Xuhang Liu, Zissimos Mourelatos

Abstract

MicroRNAs (miRNAs) are an important class of small RNAs that regulate gene expression posttranscriptionally through the microRNP (miRNP)/RNA-induced silencing complex (RISC). The core component of miRNPs is an Argonuate protein that directly binds to a miRNA. In mammals, most miRNPs are assembled through the miRNA loading complex (miRLC), which is composed of Dicer, Ago, and TRBP. miRLC processes miRNA precursors (pre-miRNAs) into miRNA duplexes and loads miRNA duplexes to Ago. Here, we describe a native gel analysis system for detecting miRNPs assembled with pre-miRNAs from mammalian lysates that ectopically express Ago2. The methods presented here provide a powerful tool for further dissecting miRNP assembly pathways in mammals.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 33%
Researcher 2 33%
Professor > Associate Professor 1 17%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 50%
Biochemistry, Genetics and Molecular Biology 1 17%
Medicine and Dentistry 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2015.
All research outputs
#14,786,093
of 22,764,165 outputs
Outputs from Methods in molecular biology
#4,675
of 13,089 outputs
Outputs of similar age
#197,785
of 352,894 outputs
Outputs of similar age from Methods in molecular biology
#295
of 995 outputs
Altmetric has tracked 22,764,165 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,894 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 995 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.

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