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Neutrophil Methods and Protocols

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Cover of 'Neutrophil Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The Role of Neutrophils in the Immune System
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    Chapter 2 Isolation of Human Neutrophils From Venous Blood
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    Chapter 3 Neutrophil Isolation From Nonhuman Species
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    Chapter 4 Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
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    Chapter 5 Rac and Rap GTPase Activation Assays
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    Chapter 6 Measurement of Phospholipid Metabolism in Intact Neutrophils
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    Chapter 7 Analysis of Protein Phosphorylation in Human Neutrophils
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    Chapter 8 Mitogen-Activated Protein Kinase Assays
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    Chapter 9 Transduction of Proteins into Intact Neutrophils
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    Chapter 10 Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
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    Chapter 11 Analysis of Electrophysiological Properties and Responses of Neutrophils
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    Chapter 12 Neutrophil Methods and Protocols
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    Chapter 13 Optical and Fluorescence Detection of Neutrophil Integrin Activation
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    Chapter 14 Analysis of Neutrophil Polarization and Chemotaxis
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    Chapter 15 Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils
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    Chapter 16 Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
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    Chapter 17 Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
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    Chapter 18 Immunofluorescence and Confocal Microscopy of Neutrophils
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    Chapter 19 Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
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    Chapter 20 Analysis of Neutrophil Membrane Traffic During Phagocytosis
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    Chapter 21 Analysis of Neutrophil Bactericidal Activity
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    Chapter 22 A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
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    Chapter 23 Measurement of Respiratory Burst Products Generated by Professional Phagocytes
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    Chapter 24 The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
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    Chapter 25 Cell-Free Assays
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    Chapter 26 Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics
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    Chapter 27 Genome-Scale Transcript Analyses in Human Neutrophils
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    Chapter 28 Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
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    Chapter 29 Detection of Intact Transcription Factors in Human Neutrophils
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    Chapter 30 Disorders of Neutrophil Function
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    Chapter 31 Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
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    Chapter 32 Diagnostic Assays for Myeloperoxidase Deficiency
Attention for Chapter 3: Neutrophil Isolation From Nonhuman Species
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Chapter title
Neutrophil Isolation From Nonhuman Species
Chapter number 3
Book title
Neutrophil Methods and Protocols
Published by
Humana Press, January 2007
DOI 10.1007/978-1-59745-467-4_3
Pubmed ID
Book ISBNs
978-1-58829-788-4, 978-1-59745-467-4
Authors

Daniel W. Siemsen, Igor A. Schepetkin, Liliya N. Kirpotina, Benfang Lei, Mark T. Quinn, Siemsen, Daniel W., Schepetkin, Igor A., Kirpotina, Liliya N., Lei, Benfang, Quinn, Mark T.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 8%
United Kingdom 1 3%
Germany 1 3%
Switzerland 1 3%
Unknown 32 84%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 34%
Student > Ph. D. Student 9 24%
Professor 4 11%
Professor > Associate Professor 4 11%
Student > Master 2 5%
Other 2 5%
Unknown 4 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 39%
Medicine and Dentistry 6 16%
Biochemistry, Genetics and Molecular Biology 4 11%
Immunology and Microbiology 4 11%
Veterinary Science and Veterinary Medicine 3 8%
Other 1 3%
Unknown 5 13%