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Stem Cell Renewal and Cell-Cell Communication

Overview of attention for book
Cover of 'Stem Cell Renewal and Cell-Cell Communication'

Table of Contents

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    Book Overview
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    Chapter 90 Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)
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    Chapter 91 Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells
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    Chapter 92 A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon
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    Chapter 93 Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche
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    Chapter 94 Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication
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    Chapter 95 Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells
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    Chapter 96 Electrophysiological Recordings from Neuroepithelial Stem Cells
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    Chapter 97 Ca 2+ Handling in Mouse Embryonic Stem Cell-Derived Cardiomyocytes
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    Chapter 98 Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer’s Disease Therapeutics
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    Chapter 119 Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells.
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    Chapter 120 In Vivo Stem Cell Transplantation Using Reduced Cell Numbers
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    Chapter 121 Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures.
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    Chapter 174 Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells
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    Chapter 176 Laboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal
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    Chapter 197 A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function
Attention for Chapter 121: Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures.
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Chapter title
Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures.
Chapter number 121
Book title
Stem Cell Renewal and Cell-Cell Communication
Published in
Methods in molecular biology, September 2014
DOI 10.1007/7651_2014_121
Pubmed ID
25224161
Book ISBNs
978-1-4939-2589-6, 978-1-4939-2590-2
Authors

Stephen Meek, Linda Sutherland, Tom Burdon, Meek S, Sutherland L, Burdon T, Meek, Stephen, Sutherland, Linda, Burdon, Tom

Abstract

The rat is one of the most commonly used laboratory animals in biomedical research and the recent isolation of genuine pluripotent rat embryonic stem (ES) cell lines has provided new opportunities for applying contemporary genetic engineering techniques to the rat and enhancing the use of this rodent in scientific research. Technical refinements that improve the stability of the rat ES cell cultures will undoubtedly further strengthen and broaden the use of these stem cells in biomedical research. Here, we describe a relatively simple and robust protocol that supports the propagation of germ line competent rat ES cells, and outline how tuning stem cell signaling using small molecule inhibitors can be used to both stabilize self-renewal of rat ES cell cultures and aid evaluation of their differentiation potential in vitro.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 13%
Unknown 7 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Researcher 2 25%
Lecturer 1 13%
Student > Master 1 13%
Unknown 2 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 50%
Biochemistry, Genetics and Molecular Biology 2 25%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2016.
All research outputs
#18,378,085
of 22,763,032 outputs
Outputs from Methods in molecular biology
#7,870
of 13,088 outputs
Outputs of similar age
#175,844
of 246,452 outputs
Outputs of similar age from Methods in molecular biology
#60
of 116 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 246,452 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 116 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.

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