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Molecular Chaperones

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Cover of 'Molecular Chaperones'

Table of Contents

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    Book Overview
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    Chapter 1 Targeted deletion of Hsf1, 2, and 4 genes in mice.
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    Chapter 2 The role of heat shock factors in stress-induced transcription.
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    Chapter 3 Hsp90 and Client Protein Maturation
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    Chapter 4 The Role of p23, Hop, Immunophilins, and Other Co-chaperones in Regulating Hsp90 Function
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    Chapter 5 Detecting HSP90 phosphorylation.
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    Chapter 6 Role of molecular chaperones in biogenesis of the protein kinome.
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    Chapter 7 Nucleotide Exchange Factors for Hsp70 Chaperones
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    Chapter 8 Reconstitution of CHIP E3 Ubiquitin Ligase Activity
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    Chapter 9 Structure-Functions of HspB1 (Hsp27).
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    Chapter 10 Combined Lentiviral and RNAi Technologies for the Delivery and Permanent Silencing of the hsp25 Gene
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    Chapter 11 Quantification of HSP27 and HSP70 Molecular Chaperone Activities
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    Chapter 12 Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
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    Chapter 13 Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
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    Chapter 14 Quantitation of Heat-Shock Proteins in Clinical Samples Using Mass Spectrometry
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    Chapter 15 Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks
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    Chapter 16 Hsp70: Anti-apoptotic and Tumorigenic Protein
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    Chapter 17 Determination of Cell Survival or Death
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    Chapter 18 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
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    Chapter 19 Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells.
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    Chapter 20 Isolation of Heat Shock Protein Complexes
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    Chapter 21 Enhancing antigen cross-presentation and T-cell priming by complexing protein antigen to recombinant large heat-shock protein.
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    Chapter 22 Investigating receptors for extracellular heat shock proteins.
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    Chapter 23 Molecular Chaperones
Attention for Chapter 9: Structure-Functions of HspB1 (Hsp27).
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Chapter title
Structure-Functions of HspB1 (Hsp27).
Chapter number 9
Book title
Molecular Chaperones
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-295-3_9
Pubmed ID
Book ISBNs
978-1-61779-294-6, 978-1-61779-295-3
Authors

André-Patrick Arrigo, Arrigo, André-Patrick

Abstract

Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20-40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation patterns of HspB1 as well as its interaction with specific partner/client polypeptides using tissue culture cells genetically modified to express different levels of this protein. The procedures have been developed in my laboratory and could be used in any well-established cellular laboratory. In addition, the different procedures presented here could be extended to test the nine other human sHsp members as well as sHsps from other species.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 24%
Student > Ph. D. Student 4 19%
Student > Master 4 19%
Student > Bachelor 2 10%
Student > Postgraduate 1 5%
Other 0 0%
Unknown 5 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 24%
Biochemistry, Genetics and Molecular Biology 3 14%
Chemistry 3 14%
Computer Science 2 10%
Mathematics 1 5%
Other 2 10%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2011.
All research outputs
#18,295,723
of 22,651,245 outputs
Outputs from Methods in molecular biology
#7,803
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Outputs of similar age
#159,884
of 180,239 outputs
Outputs of similar age from Methods in molecular biology
#166
of 229 outputs
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So far Altmetric has tracked 13,012 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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