Chapter title |
Structure-Functions of HspB1 (Hsp27).
|
---|---|
Chapter number | 9 |
Book title |
Molecular Chaperones
|
Published in |
Methods in molecular biology, January 2011
|
DOI | 10.1007/978-1-61779-295-3_9 |
Pubmed ID | |
Book ISBNs |
978-1-61779-294-6, 978-1-61779-295-3
|
Authors |
André-Patrick Arrigo, Arrigo, André-Patrick |
Abstract |
Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20-40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation patterns of HspB1 as well as its interaction with specific partner/client polypeptides using tissue culture cells genetically modified to express different levels of this protein. The procedures have been developed in my laboratory and could be used in any well-established cellular laboratory. In addition, the different procedures presented here could be extended to test the nine other human sHsp members as well as sHsps from other species. |
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