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Single-Cell Analysis

Overview of attention for book
Cover of 'Single-Cell Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction: Why Analyze Single Cells?
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    Chapter 2 Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview
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    Chapter 3 Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry
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    Chapter 4 Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts
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    Chapter 5 Single-Cell Culture in Microwells
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    Chapter 6 Isodielectric Separation and Analysis of Cells
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    Chapter 7 Single Cell Electroporation Using Microfluidic Devices
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    Chapter 8 Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
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    Chapter 9 Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
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    Chapter 10 Droplet microfluidics for single-cell analysis.
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    Chapter 11 Screening of Antigen-Specific Antibody-Secreting Cells
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    Chapter 12 Analysis of Single Eukaryotic Cells Using Raman Tweezers
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    Chapter 13 Single-Cell Microinjection Technologies
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    Chapter 14 Ultrasonic Manipulation of Single Cells
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    Chapter 15 Expanding the Horizons for Single-Cell Applications on Lab-on-a-Chip Devices
  17. Altmetric Badge
    Chapter 16 Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
Attention for Chapter 10: Droplet microfluidics for single-cell analysis.
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Chapter title
Droplet microfluidics for single-cell analysis.
Chapter number 10
Book title
Single-Cell Analysis
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-567-1_10
Pubmed ID
22323144
Book ISBNs
978-1-61779-566-4, 978-1-61779-567-1
Authors

Brouzes E, Eric Brouzes, Brouzes, Eric

Abstract

This book chapter aims at providing an overview of all the aspects and procedures needed to develop a droplet-based workflow for single-cell analysis (see Fig. 10.1). The surfactant system used to stabilize droplets is a critical component of droplet microfluidics; its properties define the type of droplet-based assays and workflows that can be developed. The scope of this book chapter is limited to fluorinated surfactant systems that have proved to generate extremely stable droplets and allow to easily retrieve the encapsulated material. The formulation section discusses how the experimental parameters influence the choice of the surfactant system to use. The circuit design section presents recipes to design and integrate different droplet modules into a whole assay. The fabrication section describes the manufacturing of microfluidic chip including the surface treatment which is pivotal in droplet microfluidics. Finally, the last section reviews the experimental setup for fluorescence detection with an emphasis on cell injection and incubation.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 41 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 21%
Researcher 8 19%
Student > Bachelor 6 14%
Student > Doctoral Student 3 7%
Other 3 7%
Other 6 14%
Unknown 7 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 29%
Biochemistry, Genetics and Molecular Biology 6 14%
Engineering 6 14%
Chemistry 5 12%
Computer Science 2 5%
Other 4 10%
Unknown 7 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 July 2014.
All research outputs
#18,375,064
of 22,758,963 outputs
Outputs from Methods in molecular biology
#7,870
of 13,089 outputs
Outputs of similar age
#199,024
of 250,042 outputs
Outputs of similar age from Methods in molecular biology
#316
of 458 outputs
Altmetric has tracked 22,758,963 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 458 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.

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