Chapter title |
Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos
|
---|---|
Chapter number | 8 |
Book title |
Wheat Biotechnology
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7337-8_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7335-4, 978-1-4939-7337-8
|
Authors |
Goetz Hensel, Cornelia Marthe, Jochen Kumlehn |
Abstract |
Methods of the Agrobacterium-mediated transformation of bread wheat (Triticum aestivum L.) have been improved in recent years so that genetic engineering can be routinely used for functional genomics as well as for wheat breeding. In the protocol described here, immature embryos of the spring-type model genotype Bobwhite SH 98 26 have been used. Preculture and temperature pretreatment of these explants have led to the reproducible generation of transgenic plants at efficiencies between 5 and 15%. Whereas primary transgenic plants regenerated in vitro commonly show reduced fitness and fertility, no apparent variations with regard to morphology and grain set in their transgenic progeny as compared to wild-type counterparts were observed. |
X Demographics
Geographical breakdown
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Germany | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 33% |
Student > Ph. D. Student | 3 | 14% |
Student > Bachelor | 3 | 14% |
Student > Master | 1 | 5% |
Student > Postgraduate | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 38% |
Biochemistry, Genetics and Molecular Biology | 5 | 24% |
Engineering | 2 | 10% |
Earth and Planetary Sciences | 1 | 5% |
Unknown | 5 | 24% |