Chapter title |
A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer.
|
---|---|
Chapter number | 3 |
Book title |
Urothelial Carcinoma
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7234-0_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7233-3, 978-1-4939-7234-0
|
Authors |
Tommasi, Stella, Besaratinia, Ahmad, Stella Tommasi, Ahmad Besaratinia |
Abstract |
Urothelial carcinoma of the bladder is one of the most common malignancies in the industrialized world, mainly caused by smoking and occupational exposure to chemicals. The favorable prognosis of early stage bladder cancer underscores the importance of early detection for the treatment of this disease. The high recurrence rate of this malignancy also highlights the need for close post-diagnosis monitoring of bladder cancer patients. As for other malignancies, aberrant DNA methylation has been shown to play a crucial role in the initiation and progression of bladder cancer, and thus holds great promise as a diagnostic and prognostic biological marker. Here, we describe a protocol for a versatile DNA methylation enrichment method, the Methylated CpG Island Recovery Assay (MIRA), which enables analysis of the DNA methylation status in individual genes or across the entire genome. MIRA is based on the ability of the methyl-binding domain (MBD) proteins, the MBD2B/MBD3L1 complex, to specifically bind methylated CpG dinucleotides. This easy-to-perform method can be used to analyze the methylome of bladder cancer or urothelial cells shed in the urine to elucidate the evolution of bladder carcinogenesis and/or identify epigenetic signatures of chemicals known to cause this malignancy. |
X Demographics
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 23% |
Student > Master | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Student > Postgraduate | 2 | 15% |
Researcher | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Nursing and Health Professions | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Materials Science | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 23% |