| Chapter title |
Detection of Cell Membrane-Bound CD46 Using Flow Cytometry.
|
|---|---|
| Chapter number | 27 |
| Book title |
The Complement System
|
| Published in |
Methods in molecular biology, November 2013
|
| DOI | 10.1007/978-1-62703-724-2_27 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-723-5, 978-1-62703-724-2
|
| Authors |
Kolev M, Kemper C, Martin Kolev, Claudia Kemper, Kolev, Martin, Kemper, Claudia |
| Abstract |
CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Professor > Associate Professor | 1 | 25% |
| Researcher | 1 | 25% |
| Student > Master | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 25% |
| Immunology and Microbiology | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |
| Unknown | 1 | 25% |