| Chapter title |
Analysis of Enzyme Activities
|
|---|---|
| Chapter number | 15 |
| Book title |
Plant Metabolic Flux Analysis
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-62703-688-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-687-0, 978-1-62703-688-7
|
| Authors |
Guillaume Ménard, Benoit Biais, Duyên Prodhomme, Patricia Ballias, Yves Gibon, Ménard, Guillaume, Biais, Benoit, Prodhomme, Duyên, Ballias, Patricia, Gibon, Yves |
| Abstract |
The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 31% |
| Professor > Associate Professor | 2 | 15% |
| Student > Master | 2 | 15% |
| Student > Ph. D. Student | 1 | 8% |
| Unspecified | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 46% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Unspecified | 1 | 8% |
| Immunology and Microbiology | 1 | 8% |
| Unknown | 3 | 23% |