Chapter title |
Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement
|
---|---|
Chapter number | 64 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, August 2017
|
DOI | 10.1007/7651_2017_64 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8747-4, 978-1-4939-8748-1
|
Authors |
Gumpper, Kristyn, Sermersheim, Matthew, Zhu, Michael X., Lin, Pei-Hui, Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, Pei-Hui Lin |
Abstract |
Muscle wasting or cachexia is commonly associated with aging and many diseases such as cancer, infection, autoimmune disorders, and trauma. Decrease in muscle mass, or muscle atrophy, is often caused by dysfunction of protein proteolytic systems, such as lysosomes, which regulate protein turnover and homeostasis. Lysosomes contain many hydrolases and proteases and, thus, represent the major organelle that control protein turnover. Recently, lysosomes have emerged as a signaling hub to integrate cellular functions of nutrient sensing and metabolism, autophagy, phagocytosis, and endocytosis, which are all related to tissue homeostasis. In this chapter, we describe the protocol used to measure lysosomal proteinase (cathepsins) activity in the skeletal muscle. A better understanding of lysosomal function in muscle homeostasis is critical in developing new therapeutic approaches to prevent muscle wasting. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 31% |
Student > Ph. D. Student | 2 | 15% |
Student > Master | 2 | 15% |
Student > Bachelor | 1 | 8% |
Unspecified | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 38% |
Agricultural and Biological Sciences | 2 | 15% |
Unspecified | 1 | 8% |
Nursing and Health Professions | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 23% |