Chapter title |
Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells
|
---|---|
Chapter number | 2 |
Book title |
Skeletal Muscle Development
|
Published in |
Methods in molecular biology, August 2017
|
DOI | 10.1007/978-1-4939-7283-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7282-1, 978-1-4939-7283-8
|
Authors |
So, Karl Kamhei, Peng, Xianlu Laura, Sun, Hao, Wang, Huating, Karl Kamhei So, Xianlu Laura Peng, Hao Sun, Huating Wang |
Abstract |
Transcriptional control of gene expression in skeletal muscle cell is involved in different processes ranging from muscle formation to regeneration. The identification of an increasing number of transcription factors, co-factors, and histone modifications has been greatly advanced by methods that allow studies of genome-wide chromatin-protein interactions. Chromatin immunoprecipitation with massively parallel DNA sequencing, or ChIP-seq, is a powerful tool for identifying binding sites of TFs/co-factors and histone modifications. The major steps of this technique involve immunoprecipitation of fragmented chromatin, followed by high-throughput sequencing to identify the protein bound regions genome-wide. Here, in this protocol, we will illustrate how the entire ChIP-seq is performed using global H3K27ac profiling in myoblast cells as an example. |
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