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Chapter title |
Generation of Endo-siRNAs in Xenopus laevis Oocytes.
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Chapter number | 3 |
Book title |
Animal Endo-SiRNAs
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Published in |
Methods in molecular biology, January 2014
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DOI | 10.1007/978-1-4939-0931-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0930-8, 978-1-4939-0931-5
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Authors |
Sammer Alnumeir, Andreas Werner |
Abstract |
Endogenous siRNAs (endo-siRNAs) are well documented and characterized in C. elegans and Drosophila. Endo-siRNAs can also be found in vertebrates; however, their biology is much less clear. They are thought to be produced by Dicer and to contribute to transposon silencing. Because of their generally low abundance and their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from Xenopus laevis, that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 27% |
Researcher | 3 | 20% |
Student > Ph. D. Student | 3 | 20% |
Student > Bachelor | 1 | 7% |
Professor | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 33% |
Agricultural and Biological Sciences | 5 | 33% |
Mathematics | 1 | 7% |
Chemistry | 1 | 7% |
Unknown | 3 | 20% |