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Animal Endo-SiRNAs

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Animal Endo-SiRNAs
Humana Press, New York, NY
Attention for Chapter 3: Generation of Endo-siRNAs in Xenopus laevis Oocytes.
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Chapter title
Generation of Endo-siRNAs in Xenopus laevis Oocytes.
Chapter number 3
Book title
Animal Endo-SiRNAs
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0931-5_3
Pubmed ID
Book ISBNs
978-1-4939-0930-8, 978-1-4939-0931-5
Authors

Sammer Alnumeir, Andreas Werner

Abstract

Endogenous siRNAs (endo-siRNAs) are well documented and characterized in C. elegans and Drosophila. Endo-siRNAs can also be found in vertebrates; however, their biology is much less clear. They are thought to be produced by Dicer and to contribute to transposon silencing. Because of their generally low abundance and their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from Xenopus laevis, that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Researcher 3 20%
Student > Ph. D. Student 3 20%
Student > Bachelor 1 7%
Professor 1 7%
Other 1 7%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 33%
Agricultural and Biological Sciences 5 33%
Mathematics 1 7%
Chemistry 1 7%
Unknown 3 20%