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Mendeley readers
Attention Score in Context
| Chapter title |
Fluorescence Polarization Assay to Quantify Protein-Protein Interactions
|
|---|---|
| Chapter number | -1592597468 |
| Book title |
Protein-Protein Interactions
|
| Published in |
Methods in molecular biology, January 2004
|
| DOI | 10.1385/1-59259-762-9:161 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-120-2, 978-1-59259-762-8
|
| Authors |
Sang-Hyun Park, Ronald T. Raines |
| Editors |
Haian Fu |
| Abstract |
A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 6% |
| Netherlands | 1 | 3% |
| Germany | 1 | 3% |
| Unknown | 32 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 25% |
| Researcher | 9 | 25% |
| Student > Bachelor | 4 | 11% |
| Student > Master | 4 | 11% |
| Student > Doctoral Student | 3 | 8% |
| Other | 3 | 8% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 33% |
| Agricultural and Biological Sciences | 8 | 22% |
| Chemistry | 6 | 17% |
| Physics and Astronomy | 2 | 6% |
| Chemical Engineering | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 4 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2014.
All research outputs
#20,231,392
of 22,757,090 outputs
Outputs from Methods in molecular biology
#9,863
of 13,089 outputs
Outputs of similar age
#130,165
of 133,076 outputs
Outputs of similar age from Methods in molecular biology
#24
of 26 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 133,076 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.