↓ Skip to main content

Drug Safety Evaluation

Overview of attention for book
Cover of 'Drug Safety Evaluation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Developing Combination Drugs in Preclinical Studies
  3. Altmetric Badge
    Chapter 2 Preclinical Evaluation of Juvenile Toxicity
  4. Altmetric Badge
    Chapter 3 Necropsy and Sampling Procedures in Rodents
  5. Altmetric Badge
    Chapter 4 Histopathology Procedures: From Tissue Sampling to Histopathological Evaluation
  6. Altmetric Badge
    Chapter 5 Principles and methods of immunohistochemistry.
  7. Altmetric Badge
    Chapter 6 Tissue Microarrays and Digital Image Analysis
  8. Altmetric Badge
    Chapter 7 Micronucleus Assay and Labeling of Centromeres with FISH Technique
  9. Altmetric Badge
    Chapter 8 The Use of Bacterial Repair Endonucleases in the Comet Assay
  10. Altmetric Badge
    Chapter 9 Manual Whole-Cell Patch-Clamping of the HERG Cardiac K + Channel
  11. Altmetric Badge
    Chapter 10 Generation and Analysis of Transcriptomics Data
  12. Altmetric Badge
    Chapter 11 Protocols of Two-Dimensional Difference Gel Electrophoresis to Investigate Mechanisms of Toxicity
  13. Altmetric Badge
    Chapter 12 Protocols and Applications of Cellular Metabolomics in Safety Studies Using Precision-Cut Tissue Slices and Carbon 13 NMR
  14. Altmetric Badge
    Chapter 13 Statistical analysis of quantitative RT-PCR results.
  15. Altmetric Badge
    Chapter 14 Evaluation of Mitochondrial Respiration in Cultured Rat Hepatocytes
  16. Altmetric Badge
    Chapter 15 FETAX Assay for Evaluation of Developmental Toxicity
  17. Altmetric Badge
    Chapter 16 Evaluation of Embryotoxicity Using the Zebrafish Model
  18. Altmetric Badge
    Chapter 17 Protocols of In Vitro Protein Covalent Binding Studies in Liver
  19. Altmetric Badge
    Chapter 18 Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro
  20. Altmetric Badge
    Chapter 19 Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro
  21. Altmetric Badge
    Chapter 20 One-Dimensional Western Blotting Coupled to LC-MS/MS Analysis to Identify Chemical-Adducted Proteins in Rat Urine
  22. Altmetric Badge
    Chapter 21 Identification of Chemical-Adducted Proteins in Urine by Multi-dimensional Protein Identification Technology (LC/LC–MS/MS)
  23. Altmetric Badge
    Chapter 22 Optimization of SELDI for Biomarker Detection in Plasma
  24. Altmetric Badge
    Chapter 23 Drug Safety Evaluation
  25. Altmetric Badge
    Chapter 24 NMR and MS methods for metabonomics.
  26. Altmetric Badge
    Chapter 25 Drug Safety Evaluation
Attention for Chapter 13: Statistical analysis of quantitative RT-PCR results.
Altmetric Badge

Mentioned by

facebook
1 Facebook page

Citations

dimensions_citation
20 Dimensions

Readers on

mendeley
12 Mendeley
citeulike
1 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Statistical analysis of quantitative RT-PCR results.
Chapter number 13
Book title
Drug Safety Evaluation
Published in
Methods in molecular biology, October 2010
DOI 10.1007/978-1-60761-849-2_13
Pubmed ID
Book ISBNs
978-1-60327-186-8, 978-1-60761-849-2
Authors

Khan-Malek R, Wang Y, Richard Khan-Malek, Ying Wang, Khan-Malek, Richard, Wang, Ying

Abstract

Real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. Yet, accurate results cannot be realized without proper statistical analysis of RT-PCR data. Here, we examine some of the issues concerning RT-PCR experiments that would benefit from rigorous statistical treatment, including normalization, quantification, efficiency estimation, and sample size calculations. Examples are used to illustrate the methods.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 17%
Unknown 10 83%

Demographic breakdown

Readers by professional status Count As %
Professor 2 17%
Student > Master 2 17%
Student > Ph. D. Student 2 17%
Student > Postgraduate 2 17%
Researcher 2 17%
Other 1 8%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 42%
Medicine and Dentistry 3 25%
Biochemistry, Genetics and Molecular Biology 1 8%
Environmental Science 1 8%
Neuroscience 1 8%
Other 0 0%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 June 2014.
All research outputs
#20,231,392
of 22,757,090 outputs
Outputs from Methods in molecular biology
#9,863
of 13,089 outputs
Outputs of similar age
#93,991
of 99,354 outputs
Outputs of similar age from Methods in molecular biology
#16
of 23 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 99,354 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.