Chapter title |
Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification
|
---|---|
Chapter number | 11 |
Book title |
Circulating Tumor Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7144-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7143-5, 978-1-4939-7144-2
|
Authors |
Nisha Kanwar, Susan J. Done, Kanwar, Nisha, Done, Susan J. |
Abstract |
Circulating tumor cells (CTCs) are a rare population of cells found in the peripheral blood of patients with many types of cancer such as breast, prostate, colon, and lung cancers. Higher numbers of these cells in blood are associated with a poorer prognosis of patients. Genomic profiling of CTCs would help characterize markers specific for the identification of these cells in blood, and also define genomic alterations that give these cells a metastatic advantage over other cells in the primary tumor. Here, we describe an immunomagnetic method to enrich CTCs from the blood of patients with breast cancer, followed by single-cell laser capture microdissection to isolate single CTCs. Whole genome amplification of isolated CTCs allows for many downstream applications to be performed to aide in their characterization, such as whole genome or exome sequencing, Single Nucleotide Polymorphism (SNP) and copy number analysis, and targeted sequencing or quantitative Polymerase Chain Reaction (qPCR) for genomic analyses. |
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Mendeley readers
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