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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe.
|
|---|---|
| Chapter number | 16 |
| Book title |
Yeast Protocols
|
| Published in |
Methods in molecular biology, May 2014
|
| DOI | 10.1007/978-1-4939-0799-1_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0798-4, 978-1-4939-0799-1
|
| Authors |
Li J, Du LL, Jun Li, Li-Lin Du |
| Abstract |
Transposon-mediated insertional mutagenesis is a powerful tool for genetic screens. We have developed a piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Here, we describe in detail the procedure for inducing and selecting transpositions, and two protocols for identifying the transposon insertion sites, one using inverse PCR to identify the insertion sites in individual mutants, and the other using high-throughput sequencing to reveal the insertion sites in a mutant pool containing hundreds to thousands of mutants. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Professor | 1 | 14% |
| Lecturer > Senior Lecturer | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Other | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Agricultural and Biological Sciences | 2 | 29% |
| Unknown | 3 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2015.
All research outputs
#18,372,841
of 22,756,196 outputs
Outputs from Methods in molecular biology
#7,865
of 13,089 outputs
Outputs of similar age
#162,985
of 226,264 outputs
Outputs of similar age from Methods in molecular biology
#43
of 111 outputs
Altmetric has tracked 22,756,196 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 111 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.