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Mammalian Synthetic Promoters

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Cover of 'Mammalian Synthetic Promoters'

Table of Contents

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    Book Overview
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    Chapter 1 Initial Considerations Before Designing a Promoter Construct
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    Chapter 2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay
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    Chapter 3 Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter
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    Chapter 4 Secreted Reporters for Monitoring Multiple Promoter Function
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    Chapter 5 Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo
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    Chapter 6 Monitoring Promoter Activity by Flow Cytometry
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    Chapter 7 Functional Screening of Core Promoter Activity
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    Chapter 8 Bioinformatically Informed Design of Synthetic Mammalian Promoters
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    Chapter 9 Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication
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    Chapter 10 Constructing Strong Cell Type-Specific Promoters Through Informed Design
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    Chapter 11 PCR Assembly of Synthetic Promoters
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    Chapter 12 The Tetracycline Responsive System
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    Chapter 13 Light-Responsive Promoters
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    Chapter 14 A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
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    Chapter 15 Promoter Activation with Electromagnetism
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    Chapter 16 Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment
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    Chapter 17 Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat
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    Chapter 18 Computational Sequence Design with R2oDNA Designer
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    Chapter 19 Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Attention for Chapter 18: Computational Sequence Design with R2oDNA Designer
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Chapter title
Computational Sequence Design with R2oDNA Designer
Chapter number 18
Book title
Mammalian Synthetic Promoters
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7223-4_18
Pubmed ID
Book ISBNs
978-1-4939-7221-0, 978-1-4939-7223-4
Authors

James T. MacDonald, Velia Siciliano

Abstract

Recently developed DNA assembly methods have enabled the rapid and simultaneous assembly of multiple parts to create complex synthetic gene circuits. A number of groups have proposed the use of computationally designed orthogonal spacer sequences to guide the ordered assembly of parts using overlap-directed or homologous recombination-based methods. This approach is particularly useful for assembling multiple parts with repetitive elements. Orthogonal spacer sequences (sometimes called UNSs-unique nucleotide sequences) also have a number of other potential uses including in the design of synthetic promoters regulated by novel regulatory elements.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 47%
Student > Ph. D. Student 2 12%
Student > Bachelor 1 6%
Student > Master 1 6%
Professor > Associate Professor 1 6%
Other 0 0%
Unknown 4 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 29%
Agricultural and Biological Sciences 4 24%
Immunology and Microbiology 2 12%
Nursing and Health Professions 1 6%
Neuroscience 1 6%
Other 1 6%
Unknown 3 18%