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Mammalian Synthetic Promoters

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Cover of 'Mammalian Synthetic Promoters'

Table of Contents

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    Book Overview
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    Chapter 1 Initial Considerations Before Designing a Promoter Construct
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    Chapter 2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay
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    Chapter 3 Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter
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    Chapter 4 Secreted Reporters for Monitoring Multiple Promoter Function
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    Chapter 5 Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo
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    Chapter 6 Monitoring Promoter Activity by Flow Cytometry
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    Chapter 7 Functional Screening of Core Promoter Activity
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    Chapter 8 Bioinformatically Informed Design of Synthetic Mammalian Promoters
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    Chapter 9 Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication
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    Chapter 10 Constructing Strong Cell Type-Specific Promoters Through Informed Design
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    Chapter 11 PCR Assembly of Synthetic Promoters
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    Chapter 12 The Tetracycline Responsive System
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    Chapter 13 Light-Responsive Promoters
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    Chapter 14 A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
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    Chapter 15 Promoter Activation with Electromagnetism
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    Chapter 16 Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment
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    Chapter 17 Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat
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    Chapter 18 Computational Sequence Design with R2oDNA Designer
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    Chapter 19 Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Attention for Chapter 15: Promoter Activation with Electromagnetism
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Chapter title
Promoter Activation with Electromagnetism
Chapter number 15
Book title
Mammalian Synthetic Promoters
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7223-4_15
Pubmed ID
Book ISBNs
978-1-4939-7221-0, 978-1-4939-7223-4
Authors

Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, Reyes S. Taméz-Guerra

Abstract

Gene expression can be modified by physical factors, such as heat, electricity and magnetic fields , and several types of ionizing and non-ionizing radiation. Promoter activation with extremely low-frequency electromagnetic fields is possible with an appropriate promoter, containing electromagnetic field response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a step-by-step guide to the assembly of a solenoid suitable for promoter activation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 29%
Student > Ph. D. Student 2 29%
Researcher 1 14%
Professor 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Medicine and Dentistry 1 14%
Unknown 2 29%