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Mendeley readers
Chapter title |
Secreted Reporters for Monitoring Multiple Promoter Function
|
---|---|
Chapter number | 4 |
Book title |
Mammalian Synthetic Promoters
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7223-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7221-0, 978-1-4939-7223-4
|
Authors |
Ghazal Lashgari, Rami S. Kantar, Bakhos A. Tannous |
Abstract |
Secreted reporter proteins are reliable modalities for monitoring of different biological processes, which can be measured longitudinally in conditioned medium of cultured cells or body fluids such as blood and urine, ex vivo. In this chapter, we will explore established secreted reporters and their applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three commonly used reporters: secreted alkaline phosphatase (SEAP ), Gaussia luciferase (Gluc), and Vargula luciferase (Vluc). |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Engineering | 1 | 33% |
Unknown | 1 | 33% |