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Mammalian Synthetic Promoters

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Cover of 'Mammalian Synthetic Promoters'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Initial Considerations Before Designing a Promoter Construct
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    Chapter 2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay
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    Chapter 3 Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter
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    Chapter 4 Secreted Reporters for Monitoring Multiple Promoter Function
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    Chapter 5 Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo
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    Chapter 6 Monitoring Promoter Activity by Flow Cytometry
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    Chapter 7 Functional Screening of Core Promoter Activity
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    Chapter 8 Bioinformatically Informed Design of Synthetic Mammalian Promoters
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    Chapter 9 Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication
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    Chapter 10 Constructing Strong Cell Type-Specific Promoters Through Informed Design
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    Chapter 11 PCR Assembly of Synthetic Promoters
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    Chapter 12 The Tetracycline Responsive System
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    Chapter 13 Light-Responsive Promoters
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    Chapter 14 A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
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    Chapter 15 Promoter Activation with Electromagnetism
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    Chapter 16 Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment
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    Chapter 17 Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat
  19. Altmetric Badge
    Chapter 18 Computational Sequence Design with R2oDNA Designer
  20. Altmetric Badge
    Chapter 19 Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Attention for Chapter 8: Bioinformatically Informed Design of Synthetic Mammalian Promoters
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Chapter title
Bioinformatically Informed Design of Synthetic Mammalian Promoters
Chapter number 8
Book title
Mammalian Synthetic Promoters
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7223-4_8
Pubmed ID
Book ISBNs
978-1-4939-7221-0, 978-1-4939-7223-4
Authors

Michael L. Roberts, Polyxeni Katsoupi, Vivian Tseveleki, Era Taoufik

Abstract

Synthetic promoters have been developed in a number of different organisms and are capable of mediating specific and enhanced levels of gene expression. Typically, cis-regulatory regions from a few genes are randomly combined to generate a synthetic promoter library, and the sequences with the highest activity are selected for in target cell lines. Here we describe a novel approach that can be employed in the construction of synthetic promoters . Specifically, we use gene expression profiles obtained from microarray datasets to select the cis-regulatory elements that comprise the synthetic promoter library. By adopting this approach, we were able to construct several promoters that could specifically mediate gene expression in colorectal cancer cells. We develop a new selection criteria based on the observed transcriptome of target cells, the frequency that identified cis-regulatory sequences occur in identified gene modules, and the length of identified cis-regulatory regions. Our method allows for the generation of synthetic promoter libraries with increased level of specificity and facilitates the selection of promoters that are highly active only under predefined gene expression profiles.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 26%
Other 2 11%
Student > Master 2 11%
Researcher 2 11%
Student > Bachelor 1 5%
Other 2 11%
Unknown 5 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 32%
Immunology and Microbiology 2 11%
Agricultural and Biological Sciences 2 11%
Engineering 2 11%
Medicine and Dentistry 1 5%
Other 1 5%
Unknown 5 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2017.
All research outputs
#15,475,586
of 22,997,544 outputs
Outputs from Methods in molecular biology
#5,381
of 13,151 outputs
Outputs of similar age
#257,283
of 421,196 outputs
Outputs of similar age from Methods in molecular biology
#468
of 1,074 outputs
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