↓ Skip to main content
What is this page? Embed badge

Mammalian Synthetic Promoters

Overview of attention for book
Cover of 'Mammalian Synthetic Promoters'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Initial Considerations Before Designing a Promoter Construct
  3. Altmetric Badge
    Chapter 2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay
  4. Altmetric Badge
    Chapter 3 Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter
  5. Altmetric Badge
    Chapter 4 Secreted Reporters for Monitoring Multiple Promoter Function
  6. Altmetric Badge
    Chapter 5 Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo
  7. Altmetric Badge
    Chapter 6 Monitoring Promoter Activity by Flow Cytometry
  8. Altmetric Badge
    Chapter 7 Functional Screening of Core Promoter Activity
  9. Altmetric Badge
    Chapter 8 Bioinformatically Informed Design of Synthetic Mammalian Promoters
  10. Altmetric Badge
    Chapter 9 Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication
  11. Altmetric Badge
    Chapter 10 Constructing Strong Cell Type-Specific Promoters Through Informed Design
  12. Altmetric Badge
    Chapter 11 PCR Assembly of Synthetic Promoters
  13. Altmetric Badge
    Chapter 12 The Tetracycline Responsive System
  14. Altmetric Badge
    Chapter 13 Light-Responsive Promoters
  15. Altmetric Badge
    Chapter 14 A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
  16. Altmetric Badge
    Chapter 15 Promoter Activation with Electromagnetism
  17. Altmetric Badge
    Chapter 16 Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment
  18. Altmetric Badge
    Chapter 17 Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat
  19. Altmetric Badge
    Chapter 18 Computational Sequence Design with R2oDNA Designer
  20. Altmetric Badge
    Chapter 19 Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Attention for Chapter 19: Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
3 tweeters

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
38 Mendeley
Summary Twitter Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Design of Synthetic Promoters for Gene Circuits in Mammalian Cells
Chapter number 19
Book title
Mammalian Synthetic Promoters
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7223-4_19
Pubmed ID
28801913
Book ISBNs
978-1-4939-7221-0, 978-1-4939-7223-4
Authors

Pratik Saxena, Daniel Bojar, Martin Fussenegger

Abstract

Synthetic biology, the synthesis of engineering and biology, has rapidly matured and has dramatically increased the complexity of artificial gene circuits in recent years. The deployment of intricate synthetic gene circuits in mammalian cells requires the establishment of very precise and orthogonal control of transgene expression. In this chapter, we describe methods of modulating the expression of transgenes at the transcriptional level. Using cAMP-response element-binding protein (CREB)-dependent promoters as examples, a tool for the precise tuning of gene expression by using different core promoters and by varying the binding affinity of transcription factor operator sites is described.

View on publisher site

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 21%
Researcher 8 21%
Student > Bachelor 4 11%
Student > Master 2 5%
Professor 1 3%
Other 3 8%
Unknown 12 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 34%
Agricultural and Biological Sciences 4 11%
Chemical Engineering 2 5%
Medicine and Dentistry 2 5%
Neuroscience 2 5%
Other 1 3%
Unknown 14 37%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2018.
All research outputs
#15,475,586
of 22,997,544 outputs
Outputs from Methods in molecular biology
#5,381
of 13,151 outputs
Outputs of similar age
#257,283
of 421,196 outputs
Outputs of similar age from Methods in molecular biology
#468
of 1,074 outputs
Altmetric has tracked 22,997,544 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,196 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.