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Mendeley readers
Chapter title |
Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases
|
---|---|
Chapter number | 7 |
Book title |
ErbB Receptor Signaling
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7219-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7218-0, 978-1-4939-7219-7
|
Authors |
Babak Nami, Zhixiang Wang |
Abstract |
Immunofluorescence staining is an effective method for visualizing ErbB family of receptor tyrosine kinases and to monitor their expression, phosphorylation, and localization in individual cells. However, immunofluorescence staining for membrane proteins is required preserving integrity of cell membrane intact by protecting the cells from organic solvents. Permeabilization of cells may increase nonspecific intracellular background signal as an inevitable result. This chapter describes procedures for the application of indirect immunofluorescence staining for ErbB receptors in adherent and suspended cells as well as in paraffin-embedded tissue sections. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Unknown | 4 | 80% |