| Chapter title |
Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem Using MorphoGraphX.
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Cell Morphogenesis
|
| Published in |
Methods in molecular biology, October 2013
|
| DOI | 10.1007/978-1-62703-643-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-642-9, 978-1-62703-643-6
|
| Authors |
de Reuille PB, Robinson S, Smith RS, Pierre Barbier de Reuille, Sarah Robinson, Richard S. Smith |
| Abstract |
Confocal microscopy is a technique widely used to live-image plant tissue. Cells can be visualized by using fluorescent probes that mark the cell wall or plasma membrane. This enables the confocal microscope to be used as a 3D scanner with submicron precision. Here we present a protocol using the 3D image processing software MorphoGraphX (http://www.MorphoGraphX.org) to extract the surface geometry and cell shapes in the shoot apex. By segmenting cells over consecutive time points, precise growth maps of the shoot apex can be produced. It is also possible to tag a protein of interest with a fluorescent marker and quantify protein expression at the cellular level. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 32% |
| Researcher | 5 | 23% |
| Student > Master | 3 | 14% |
| Professor > Associate Professor | 2 | 9% |
| Lecturer | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 59% |
| Biochemistry, Genetics and Molecular Biology | 4 | 18% |
| Computer Science | 2 | 9% |
| Unknown | 3 | 14% |