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Cancer cell culture

Overview of attention for book
Cover of 'Cancer cell culture'

Table of Contents

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    Book Overview
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    Chapter 1 Cancer Biology
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    Chapter 2 Principles of cancer cell culture.
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    Chapter 3 Storage of Cell Lines
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    Chapter 4 Characterization and Authentication of Cancer Cell Lines: An Overview
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    Chapter 5 Online Verification of Human Cell Line Identity by STR DNA Typing
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    Chapter 6 Cytogenetic Analysis of Cancer Cell Lines
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    Chapter 7 Cell Culture Contamination
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    Chapter 8 Detecting mycoplasma contamination in cell cultures by polymerase chain reaction.
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    Chapter 9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics
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    Chapter 10 Quality Assurance and Good Laboratory Practice
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    Chapter 11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning
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    Chapter 12 Isolation and Culture of Colon Cancer Cells and Cell Lines
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    Chapter 13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
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    Chapter 14 Isolation and Culture of Squamous Cell Carcinoma Lines
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    Chapter 15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines
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    Chapter 16 Establishment and Culture of Leukemia–Lymphoma Cell Lines
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    Chapter 17 Isolation of Inflammatory Cells from Human Tumours
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    Chapter 18 Isolation of Endothelial Cells from Human Tumors
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    Chapter 19 Cellular Chemosensitivity Assays: An Overview
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    Chapter 20 Cell sensitivity assays: the MTT assay.
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    Chapter 21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
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    Chapter 22 Differential Staining Cytotoxicity Assay: A Review
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    Chapter 23 Real-time cytotoxicity assays.
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    Chapter 24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
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    Chapter 25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay
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    Chapter 26 Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR
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    Chapter 27 Cell Migration and Invasion Assays
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    Chapter 28 Angiogenesis Assays
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    Chapter 29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
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    Chapter 30 Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines
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    Chapter 31 Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transcriptase Polymerase Chain Reaction
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    Chapter 32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins
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    Chapter 33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated Immunotherapy
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    Chapter 34 Analysis of Drug Interactions
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    Chapter 35 Transfection and DNA-Mediated Gene Transfer
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    Chapter 36 Drug Design and Testing: Profiling of Antiproliferative Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]-Thymidine Incorporation Assay
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    Chapter 37 Feeder Layers: Co-culture with Nonneoplastic Cells
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    Chapter 38 Xenotransplantation of Breast Cancers
Attention for Chapter 20: Cell sensitivity assays: the MTT assay.
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Chapter title
Cell sensitivity assays: the MTT assay.
Chapter number 20
Book title
Cancer Cell Culture
Published in
Methods in molecular biology, December 2010
DOI 10.1007/978-1-61779-080-5_20
Pubmed ID
21516412
Book ISBNs
978-1-61779-079-9, 978-1-61779-080-5
Authors

van Meerloo J, Kaspers GJ, Cloos J, Johan van Meerloo, Gertjan J. L. Kaspers, Jacqueline Cloos, Meerloo, Johan van, Kaspers, Gertjan J. L., Cloos, Jacqueline, Meerloo, Johan

Abstract

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro cytotoxic effects of drugs on cell lines or primary patient cells. In this chapter the protocol of the assay is described including important considerations relevant for each step of the assay as well as its limitations and possible applications.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 580 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 3 <1%
United Kingdom 3 <1%
Malaysia 2 <1%
Brazil 2 <1%
Netherlands 1 <1%
Pakistan 1 <1%
Austria 1 <1%
Czechia 1 <1%
Hong Kong 1 <1%
Other 4 <1%
Unknown 561 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 201 35%
Student > Master 186 32%
Student > Ph. D. Student 175 30%
Researcher 69 12%
Student > Doctoral Student 51 9%
Other 128 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 197 34%
Agricultural and Biological Sciences 148 26%
Pharmacology, Toxicology and Pharmaceutical Science 102 18%
Chemistry 97 17%
Medicine and Dentistry 94 16%
Other 137 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2015.
All research outputs
#18,371,959
of 22,755,127 outputs
Outputs from Methods in molecular biology
#7,865
of 13,089 outputs
Outputs of similar age
#159,868
of 180,157 outputs
Outputs of similar age from Methods in molecular biology
#166
of 229 outputs
Altmetric has tracked 22,755,127 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,157 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 229 others from the same source and published within six weeks on either side of this one. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.

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