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Mendeley readers
Attention Score in Context
| Chapter title |
Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR
|
|---|---|
| Chapter number | 23 |
| Book title |
Host-Fungus Interactions
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-539-8_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-538-1, 978-1-61779-539-8
|
| Authors |
David L. Moyes, Julian R. Naglik |
| Editors |
Alexandra C. Brand, Donna M. MacCallum |
| Abstract |
Intracellular responses to external pathogens/stimuli are crucial to the host's response to infection. The methods used to analyse these responses fall into many categories. Activation of proteins as part of a signal cascade can be screened for using conventional immunoblotting techniques or immunoprecipitation to identify the presence of modified proteins or protein complexes. Transcription factor activity can be screened for by EMSA or ELISAs to identify DNA binding of these factors. Finally, expression of activated genes can be quantified using real-time PCR methods. Here, we will show how to perform these assays and discuss the relative merits of each. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 50% |
| Student > Bachelor | 2 | 17% |
| Lecturer | 2 | 17% |
| Student > Ph. D. Student | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 25% |
| Medicine and Dentistry | 2 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Immunology and Microbiology | 1 | 8% |
| Neuroscience | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 2 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2017.
All research outputs
#2,932,656
of 22,753,345 outputs
Outputs from Methods in molecular biology
#589
of 13,089 outputs
Outputs of similar age
#24,049
of 244,432 outputs
Outputs of similar age from Methods in molecular biology
#39
of 468 outputs
Altmetric has tracked 22,753,345 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 244,432 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 468 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.