Chapter title |
PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.
|
---|---|
Chapter number | 3 |
Book title |
Homing Endonucleases
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-62703-968-0_3 |
Pubmed ID | |
Book ISBNs |
978-1-62703-967-3, 978-1-62703-968-0
|
Authors |
Hafez M, Guha TK, Shen C, Sethuraman J, Hausner G, Mohamed Hafez, Tuhin Kumar Guha, Chen Shen, Jyothi Sethuraman, Georg Hausner, Hafez, Mohamed, Guha, Tuhin Kumar, Shen, Chen, Sethuraman, Jyothi, Hausner, Georg |
Abstract |
Fungal mitochondrial genomes act as "reservoirs" for homing endonucleases. These enzymes with their DNA site-specific cleavage activities are attractive tools for genome editing and gene therapy applications. Bioprospecting and characterization of naturally occurring homing endonucleases offers an alternative to synthesizing artificial endonucleases. Here, we describe methods for PCR-based screening of fungal mitochondrial rRNA genes for homing endonuclease encoding sequences, and we also provide protocols for the purification and biochemical characterization of putative native homing endonucleases. |
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