Chapter title |
Next-generation whole genome sequencing of dengue virus.
|
---|---|
Chapter number | 12 |
Book title |
Dengue
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0348-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0347-4, 978-1-4939-0348-1
|
Authors |
Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M Sessions, Martin Lloyd Hibberd, October M. Sessions, Aw, Pauline Poh Kim, Sessions, Paola Florez, Wilm, Andreas, Hoang, Long Truong, Nagarajan, Niranjan, Sessions, October M., Hibberd, Martin Lloyd, Sessions, Paola Florez de |
Abstract |
RNA viruses are notorious for their ability to quickly adapt to selective pressure from the host immune system and/or antivirals. This adaptability is likely due to the error-prone characteristics of their RNA-dependent, RNA polymerase [1, 2]. Dengue virus, a member of the Flaviviridae family of positive-strand RNA viruses, is also known to share these error-prone characteristics [3]. Utilizing high-throughput, massively parallel sequencing methodologies, or next-generation sequencing (NGS), we can now accurately quantify these populations of viruses and track the changes to these populations over the course of a single infection. The aim of this chapter is twofold: to describe the methodologies required for sample preparation prior to sequencing and to describe the bioinformatics analyses required for the resulting data. |
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Mendeley readers
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