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Microbial Biofilms

Overview of attention for book
Cover of 'Microbial Biofilms'

Table of Contents

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    Book Overview
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    Chapter 1 Methods for Dynamic Investigations of Surface-Attached In Vitro Bacterial and Fungal Biofilms
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    Chapter 2 Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms
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    Chapter 3 Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and Enterococcal fsr Systems
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    Chapter 4 Advanced Techniques for In Situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy
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    Chapter 5 Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyze Multispecies Oral Biofilms
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    Chapter 6 Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections
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    Chapter 7 Experimental Approaches to Investigating the Vaginal Biofilm Microbiome
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    Chapter 8 Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopedic Infections
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    Chapter 9 Animal Models to Evaluate Bacterial Biofilm Development
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    Chapter 10 Animal models to investigate fungal biofilm formation.
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    Chapter 11 Nonmammalian model systems to investigate fungal biofilms.
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    Chapter 12 Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors
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    Chapter 13 In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
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    Chapter 14 Biofilm Matrix-Degrading Enzymes
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    Chapter 15 Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices
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    Chapter 16 Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy
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    Chapter 17 Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa In Vitro and In Vivo
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    Chapter 18 Contribution of confocal laser scanning microscopy in deciphering biofilm tridimensional structure and reactivity.
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    Chapter 19 Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents.
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    Chapter 20 Bacteriophage Attack as an Anti-biofilm Strategy
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    Chapter 21 Photodynamic therapy as a novel antimicrobial strategy against biofilm-based nosocomial infections: study protocols.
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    Chapter 22 Capturing Air–Water Interface Biofilms for Microscopy and Molecular Analysis
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    Chapter 23 Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic Analyses
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    Chapter 24 Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Stone Cultural Heritage and Frescoes
  26. Altmetric Badge
    Chapter 25 Microbial Biofilms
Attention for Chapter 10: Animal models to investigate fungal biofilm formation.
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Chapter title
Animal models to investigate fungal biofilm formation.
Chapter number 10
Book title
Microbial Biofilms
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-4939-0467-9_10
Pubmed ID
Book ISBNs
978-1-4939-0466-2, 978-1-4939-0467-9
Authors

Chandra J, Pearlman E, Ghannoum MA, Jyotsna Chandra, Eric Pearlman, Mahmoud A. Ghannoum

Abstract

Microbial biofilms play an essential role in several infectious diseases and are defined as extensive communities of sessile organisms irreversibly associated with a surface, encased within a polysaccharide-rich extracellular matrix (ECM), and exhibiting enhanced resistance to antimicrobial drugs. Forming a biofilm provides the microbes protection from environmental stresses due to contaminants, nutritional depletion, or imbalances, but is dangerous to human health due to their inherent robustness and elevated resistance.The use of indwelling medical devices (e.g., central venous catheters, CVCs) in current therapeutic practice is associated with 80-90 % of hospital-acquired bloodstream and deep tissue infections. Most cases of catheter-related bloodstream infections (CRBSIs) involve colonization of microorganisms on catheter surfaces where they form a biofilm. Additionally, Fusarium solani and F. oxysporum were the causative organisms of the 2005/2006 outbreak of contact lens-associated fungal keratitis in the United States, Europe, the UK, and Singapore, and these infections involved formation of biofilms on contact lens. Fungal biofilm formation is studied using a number of techniques, involving the use of a wide variety of substrates and growth conditions. In vitro techniques involving the use of confocal scanning laser/scanning electron microscopy, metabolic activity assay, dry weight measurements, and antifungal susceptibility assays are increasingly used by investigators to quantify and evaluate biofilm morphology. However, there are not many in vivo models used to validate biofilm-associated infections. In this protocol, we describe a clinically relevant rabbit model of C. albicans biofilm-associated catheter infection to evaluate the morphology, topography, and architecture of fungal biofilms. We also describe a murine model of contact lens-associated Fusarium keratitis.Evaluation of the formation of fungal biofilms on catheters in vivo, their analysis using scanning electron microscopy (SEM) and quantitative catheter culture (QCC), and treatment of biofilms using antimicrobial lock therapy can be completed in ~20-25 days using the described methods. The rabbit model has utility in evaluating the efficacy of lock solutions. In addition, the murine model of contact lens-associated Fusarium keratitis enables characterizing/comparing the formation of Fusarium biofilms on contact lenses in vitro and determining their role in vivo.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 29%
Student > Ph. D. Student 6 21%
Researcher 3 11%
Student > Doctoral Student 2 7%
Student > Postgraduate 2 7%
Other 2 7%
Unknown 5 18%
Readers by discipline Count As %
Medicine and Dentistry 11 39%
Biochemistry, Genetics and Molecular Biology 5 18%
Agricultural and Biological Sciences 3 11%
Nursing and Health Professions 2 7%
Earth and Planetary Sciences 1 4%
Other 0 0%
Unknown 6 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2014.
All research outputs
#20,226,756
of 22,751,628 outputs
Outputs from Methods in molecular biology
#9,859
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Outputs of similar age
#192,070
of 224,543 outputs
Outputs of similar age from Methods in molecular biology
#94
of 148 outputs
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