Chapter title |
Identification and Characterization of N-Glycosylated Proteins Using Proteomics
|
---|---|
Chapter number | 17 |
Book title |
Functional Proteomics
|
Published in |
Methods in molecular biology, January 2008
|
DOI | 10.1007/978-1-59745-398-1_17 |
Pubmed ID | |
Book ISBNs |
978-1-58829-971-0, 978-1-59745-398-1
|
Authors |
David S. Selby, Martin R. Larsen, Cosima Damiana Calvano, Ole Nørregaard Jensen |
Abstract |
Glycoproteins constitute a large fraction of the proteome. The fundamental role of protein glycosylation in cellular development, growth, and differentiation, tissue development, and in host-pathogen interactions is by now widely accepted. Proteome-wide characterization of glycoproteins is a complex task and is currently achieved by mass spectrometry-based methods that enable identification of glycoproteins and localization, classification, and analysis of individual glycan structures on proteins. In this chapter we briefly introduce a range of analytical technologies for recovery and analysis of glycoproteins and glycopeptides. Combinations of affinity-enrichment techniques, chemical and biochemical protocols, and advanced mass spectrometry facilitate detailed glycoprotein analysis in proteomics, from fundamental biological studies to biomarker discovery in biomedicine. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 33% |
Student > Doctoral Student | 3 | 17% |
Student > Master | 3 | 17% |
Professor | 2 | 11% |
Researcher | 2 | 11% |
Other | 1 | 6% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 33% |
Biochemistry, Genetics and Molecular Biology | 4 | 22% |
Chemistry | 4 | 22% |
Engineering | 1 | 6% |
Unknown | 3 | 17% |