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2D PAGE: Sample Preparation and Fractionation

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Cover of '2D PAGE: Sample Preparation and Fractionation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Mechanical/physical methods of cell disruption and tissue homogenization.
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    Chapter 2 Bacteria and Yeast Cell Disruption Using Lytic Enzymes
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    Chapter 3 Sample Solublization Buffers for Two-Dimensional Electrophoresis
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    Chapter 4 Quantitation of Protein in Samples Prepared for 2-D Electrophoresis
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    Chapter 5 Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis
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    Chapter 6 Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction
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    Chapter 7 Difference Gel Electrophoresis Based on Lys/Cys Tagging
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    Chapter 8 Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine
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    Chapter 9 Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
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    Chapter 10 ICPL—Isotope-Coded Protein Label
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    Chapter 11 Radiolabeling for Two-Dimensional Gel Analysis
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    Chapter 12 Sequential Extraction of Proteins by Chemical Reagents
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    Chapter 13 Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg
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    Chapter 14 Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis
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    Chapter 15 Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns
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    Chapter 16 Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures
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    Chapter 17 Fractionation of Proteins by Immobilized Metal Affinity Chromatography
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    Chapter 18 Fractionation of Proteins by Heparin Chromatography
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    Chapter 19 Fractionation of complex protein mixtures by liquid-phase isoelectric focusing.
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    Chapter 20 Microscale Isoelectric Focusing in Solution
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    Chapter 21 2D PAGE: Sample Preparation and Fractionation
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    Chapter 22 Sample Prefractionation in Granulated Sephadex IEF Gels
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    Chapter 23 Free-Flow Electrophoresis System for Plasma Proteomic Applications
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    Chapter 24 Protein Fractionation by Preparative Electrophoresis
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    Chapter 25 2D PAGE: Sample Preparation and Fractionation
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    Chapter 26 Isolation of Highly Pure Rat Liver Mitochondria with the Aid of Zone-Electrophoresis in a Free Flow Device (ZE-FFE)
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    Chapter 27 Isolation of Proteins and Protein Complexes by Immunoprecipitation
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    Chapter 28 Isolation of Phosphoproteins
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    Chapter 29 Glycoprotein Enrichment Through Lectin Affinity Techniques
  31. Altmetric Badge
    Chapter 30 Isolation of bacterial cell membranes proteins using carbonate extraction.
  32. Altmetric Badge
    Chapter 31 Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems
  33. Altmetric Badge
    Chapter 32 The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis
  34. Altmetric Badge
    Chapter 33 Isolation of Membrane Protein Complexes by Blue Native Electrophoresis
  35. Altmetric Badge
    Chapter 34 Tissue Microdissection
Attention for Chapter 18: Fractionation of Proteins by Heparin Chromatography
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Chapter title
Fractionation of Proteins by Heparin Chromatography
Chapter number 18
Book title
2D PAGE: Sample Preparation and Fractionation
Published in
Methods in molecular biology, January 2008
DOI 10.1007/978-1-60327-064-9_18
Pubmed ID
Book ISBNs
978-1-58829-722-8, 978-1-60327-064-9
Authors

Sheng Xiong, Ling Zhang, Qing-Yu He, Xiong, Sheng, Zhang, Ling, He, Qing-Yu

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 <1%
Ireland 1 <1%
Unknown 112 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 22%
Student > Master 22 19%
Student > Bachelor 17 15%
Researcher 14 12%
Student > Doctoral Student 3 3%
Other 9 8%
Unknown 24 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 37 32%
Biochemistry, Genetics and Molecular Biology 30 26%
Chemistry 8 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Engineering 2 2%
Other 6 5%
Unknown 29 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2017.
All research outputs
#7,534,266
of 22,988,380 outputs
Outputs from Methods in molecular biology
#2,334
of 13,149 outputs
Outputs of similar age
#42,007
of 156,936 outputs
Outputs of similar age from Methods in molecular biology
#31
of 87 outputs
Altmetric has tracked 22,988,380 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,149 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,936 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.