Chapter title |
Characterization of Cell-Type-Specific DNA Binding Sites of Plant Transcription Factors Using Chromatin Immunoprecipitation
|
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Chapter number | 4 |
Book title |
Plant Gene Regulatory Networks
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Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7125-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7124-4, 978-1-4939-7125-1
|
Authors |
Lau, On Sun, On Sun Lau |
Editors |
Kerstin Kaufmann, Bernd Mueller-Roeber |
Abstract |
The generation of diverse cell types in multicellular organisms often requires the activity of cell-type-specific transcription factors. Understanding where these transcription factors bind in controlling specific cellular programs is critical. However, probing these cell-type-specific factors in vivo with standard chromatin immunoprecipitation (ChIP) assays remains a challenge. We have developed an optimized ChIP assay termed Maximized Objects for Better Enrichment (MOBE)-ChIP, which improves ChIP sensitivity and allows the detection of cell-type-specific signals at a genome-wide scale. Here, I describe the procedure for implementing this method for the study of plant transcription factors. Besides being useful for cell-type-specific studies, MOBE-ChIP can also be employed as a general strategy for enhancing ChIP signals. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 50% |
Student > Postgraduate | 1 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 100% |