| Chapter title |
Long-PCR amplification of human genomic DNA.
|
|---|---|
| Chapter number | 6 |
| Book title |
PCR Mutation Detection Protocols
|
| Published in |
Methods in molecular biology, January 2011
|
| DOI | 10.1007/978-1-60761-947-5_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-946-8, 978-1-60761-947-5
|
| Authors |
Keeney S, Stephen Keeney, Keeney, Stephen |
| Abstract |
Standard polymerase chain reaction (PCR) protocols amplify relatively small fragments precluding the use of this approach when examining gross rearrangements of DNA. By using combinations of DNA polymerases, which feature either good polymerase activity or error-correction abilities, it is now possible to extend the length of DNA fragment that can be amplified. These "long-PCR" protocols have allowed the development of more rapid and convenient ways to analyse large-scale rearrangements of DNA and in many cases has superseded alternative approaches such as Southern blotting. The protocol described in this chapter illustrates some of the key points to be considered when developing a long PCR protocol. |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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|---|---|---|
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| Lecturer | 1 | 7% |
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| Unknown | 6 | 40% |
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|---|---|---|
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| Unknown | 6 | 40% |