Chapter title |
High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Plasma by LC-MS
|
---|---|
Chapter number | 14 |
Book title |
Serum/Plasma Proteomics
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7057-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7056-8, 978-1-4939-7057-5
|
Authors |
Nicolas Christinat, Delphine Morin-Rivron, Mojgan Masoodi |
Editors |
David W. Greening, Richard J. Simpson |
Abstract |
Nonesterified fatty acids are important biological molecules which have multiple functions such as energy storage, gene regulation, or cell signaling. Comprehensive profiling of nonesterified fatty acids in biofluids can facilitate studying and understanding their roles in biological systems. For these reasons, we have developed and validated a high-throughput, nontargeted lipidomics method coupling liquid chromatography to high-resolution mass spectrometry for quantitative analysis of nonesterified fatty acids. Sufficient chromatographic separation is achieved to separate positional isomers such as polyunsaturated and branched-chain species and quantify a wide range of nonesterified fatty acids in human plasma samples. However, this method is not limited only to these fatty acid species and offers the possibility to perform untargeted screening of additional nonesterified fatty acid species. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 13% |
Researcher | 3 | 13% |
Student > Master | 3 | 13% |
Professor | 1 | 4% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 48% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 13% |
Agricultural and Biological Sciences | 3 | 13% |
Chemistry | 2 | 9% |
Medicine and Dentistry | 2 | 9% |
Nursing and Health Professions | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 48% |