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Homing Endonucleases

Overview of attention for book
Cover of 'Homing Endonucleases'

Table of Contents

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    Book Overview
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    Chapter 1 Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers
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    Chapter 2 Bioinformatic identification of homing endonucleases and their target sites.
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    Chapter 3 PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.
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    Chapter 4 Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein
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    Chapter 5 Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE
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    Chapter 6 PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis
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    Chapter 7 A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases.
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    Chapter 8 Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection.
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    Chapter 9 A Yeast-Based Recombination Assay for Homing Endonuclease Activity
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    Chapter 10 Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile
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    Chapter 11 Quantifying the information content of homing endonuclease target sites by single base pair profiling.
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    Chapter 12 Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries.
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    Chapter 13 Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
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    Chapter 14 Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes.
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    Chapter 15 Bioinformatics identification of coevolving residues.
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    Chapter 16 Identification and Analysis of Genomic Homing Endonuclease Target Sites
  18. Altmetric Badge
    Chapter 17 Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
Attention for Chapter 13: Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

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Chapter title
Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
Chapter number 13
Book title
Homing Endonucleases
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-968-0_13
Pubmed ID
24510268
Book ISBNs
978-1-62703-967-3, 978-1-62703-968-0
Authors

Kyle Jacoby, Andrew M. Scharenberg, Jacoby, Kyle, Scharenberg, Andrew M.

Abstract

Knowing the target sequence of a DNA-binding protein is vital in obtaining fundamental characteristics of the protein and evaluating properties of the protein-DNA interaction. For example, novel homing endonucleases cannot be proven to be functional until a predicted target site is tested. Unfortunately, target site prediction is not always easy, or even possible, depending on the amount of sequence data available. Here we describe a modification of SELEX using yeast surface display that can quickly and inexpensively resolve DNA-binding targets in high throughput for proteins without any prior assumptions or knowledge regarding the target site. This protocol is easily integrated into the yeast surface display pipeline and is leveraged by the expansive number of existing tools for both SELEX and yeast surface display.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 33%
Researcher 1 17%
Student > Postgraduate 1 17%
Student > Master 1 17%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Agricultural and Biological Sciences 2 33%
Chemistry 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2014.
All research outputs
#15,294,762
of 22,745,803 outputs
Outputs from Methods in molecular biology
#5,313
of 13,090 outputs
Outputs of similar age
#189,979
of 305,223 outputs
Outputs of similar age from Methods in molecular biology
#200
of 597 outputs
Altmetric has tracked 22,745,803 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,223 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.

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