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Plant Gene Regulatory Networks

Overview of attention for book
Cover of 'Plant Gene Regulatory Networks'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
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    Chapter 2 Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis
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    Chapter 3 Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting
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    Chapter 4 Characterization of Cell-Type-Specific DNA Binding Sites of Plant Transcription Factors Using Chromatin Immunoprecipitation
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    Chapter 5 Yeast One- and Two-Hybrid High-Throughput Screenings Using Arrayed Libraries
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    Chapter 6 SELEX-Seq: A Method to Determine DNA Binding Specificities of Plant Transcription Factors
  8. Altmetric Badge
    Chapter 7 Analysis of a Plant Transcriptional Regulatory Network Using Transient Expression Systems
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    Chapter 8 Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors
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    Chapter 9 Characterization of Mediator Complex and its Associated Proteins from Rice
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    Chapter 10 DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing
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    Chapter 11 In Situ Hi-C Library Preparation for Plants to Study Their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale
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    Chapter 12 Multiplexed Transcriptional Activation or Repression in Plants Using CRISPR-dCas9-Based Systems
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    Chapter 13 Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants
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    Chapter 14 Design of Knowledge Bases for Plant Gene Regulatory Networks
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    Chapter 15 AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species
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    Chapter 16 Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development
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    Chapter 17 Predicting Transcription Factor Binding Sites and Their Cognate Transcription Factors Using Gene Expression Data
  19. Altmetric Badge
    Chapter 18 Computational Approaches to Study Gene Regulatory Networks
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    Chapter 19 Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation, and Prediction
  21. Altmetric Badge
    Chapter 20 ODE-Based Modeling of Complex Regulatory Circuits
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    Chapter 21 Inferring Gene Regulatory Networks in the Arabidopsis Root Using a Dynamic Bayesian Network Approach
Attention for Chapter 5: Yeast One- and Two-Hybrid High-Throughput Screenings Using Arrayed Libraries
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Chapter title
Yeast One- and Two-Hybrid High-Throughput Screenings Using Arrayed Libraries
Chapter number 5
Book title
Plant Gene Regulatory Networks
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7125-1_5
Pubmed ID
28623579
Book ISBNs
978-1-4939-7124-4, 978-1-4939-7125-1
Authors

Sánchez-Montesino, Rocío, Oñate-Sánchez, Luis, Rocío Sánchez-Montesino, Luis Oñate-Sánchez

Editors

Kerstin Kaufmann, Bernd Mueller-Roeber

Abstract

Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/Y2H) have been used by many laboratories to detect DNA-protein (Y1H) and protein-protein interactions (Y2H). These systems use yeast cells (Saccharomyces cerevisiae) as a eukaryotic "test tube" and are amenable for most labs in the world. The development of highly efficient cloning methods has fostered the generation of large collections of open reading frames (ORFs) for several organisms that have been used for yeast screenings. Here, we describe a simple mating based method for high-throughput screenings of arrayed ORF libraries with DNA (Y1H) or protein (Y2H) baits not requiring robotics. One person can easily carry out this protocol in approximately 10 h of labor spread over 5 days. It can also be scaled down to test one-to-one (few) interactions, scaled up (i.e., robotization) and is compatible with several library formats (i.e., 96, 384-well microtiter plates).

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 25%
Researcher 4 20%
Student > Doctoral Student 3 15%
Student > Ph. D. Student 3 15%
Student > Master 2 10%
Other 0 0%
Unknown 3 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 35%
Agricultural and Biological Sciences 7 35%
Materials Science 1 5%
Unknown 5 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2017.
All research outputs
#18,555,330
of 22,981,247 outputs
Outputs from Methods in molecular biology
#7,946
of 13,149 outputs
Outputs of similar age
#241,820
of 316,926 outputs
Outputs of similar age from Methods in molecular biology
#170
of 280 outputs
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So far Altmetric has tracked 13,149 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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